Genome-specific Molecular Marker Selection In Triticeae (Poaceae)

The tribe Triticeae includes23basic genomes in diploid species. The diploid species through natural hybrid formed polyploidy species. There are still many questions in Triticeae species, such as the phylogenetic relationships among species and genera, the origin of polyploidy species, and the origin of unknown genomes. For detecting foreign genetic in distant hybridization breeding, it is necessary for finding the genome specific DNA fragments.Variations occurred in different genomes in Triticeae based on their RPB2and Acc1gene sequence. In the present study,28pairs of genome-specific primers were designed according to the RPB2and Acc1gene sequences, respectively.23diploid species representing19different genomes in Triticeae and two tetraploid species (StE) were used for exploring genome-specific markers. The main results as following:(1) Ee genome specific primers (F18/R18) produced a band with105bp in Ee-containing species and have no band in species with the Eb genome.(2) H-and P-genome specific primers (P-F2/R2and H-F2/R2) produced a band with600bp in species with the St、Ns、P、H、V、W、Ee genomes, respectively.(3) St-, W-、V-and E genome-specific primers (St-F2/R2、W-F2/R2、V-F2/R2、E-F3/R3) produced a band with600bp in all species with different genome.(4) St-and Ns-genome specific primers (St-F3/R3、Ns-F1/R1) could not produce St-and Ns-genome specific bands in all species used in this study. (5) Ee-genome specific primers (F19/R19and F22/R22) produced a band with400bp in all species used in this study.In conclusion, we find an Ee-genome specific band, which could be used for identifying the Ee-genome specific species in Triticeae.