Differential Expression Analysis Of Protein In Transgenic AtCIPK23Potato(Solanum Tuberosum) In Responsing To Low Potassium Stress

potassium deficiency in soil of crop production constraints, especially for potash crop potatoes, increase its potassium absorption efficiency is effective means of addressing this problem. This project group early has got the AtCIPK23transgenic potato several strains Department, and proved its has resistance low potassium characteristics, protein kinase AtCIPK23participation intends South mustard CBL1/9-AtCIPK23-AKT1between guide potassium absorption signal pathway, however outside source AtCIPK23gene improve potato low potassium tolerance sexual specific role mechanism and gene into by caused of unknown effect is not clearly, this experimental tries to using protein group learn to research these problem. Use common TCA-acetone precipitation and phenol extraction method to extract protein of potato plantlets, experimental comparison of two-dimensional electropho-resis revealed that can get better2-DE maps by TCA-acetone precipitation method, more suitable for proteomic analysis of potato plantlets.For research transgenic potato resistance low potassium mechanism, select wild type E3and transgenic-AtCIPK23type strains L34for research, respectively to1/2MS nutrition liquid and low potassium nutrition liquid48h hydroponics processing, on group culture seedlings ground part and underground part based, for Two-dimensional gel electrophoresis experiment, through analysis wild type and transgenic type protein expression Atlas of analysis, filter35a significantly differences protein points for MALDI-TOF-TOF MS mass spectrum identification. Analysis indicates that low potassium stress led to potato strains of energy metabolism related protein expression volume cut, on the low potassium stress increased has strains of of oxidation stress pressure, caused wild type strains of ethanol dehydrogenation enzyme, and methyl sulfur ammonia acid synthesis enzyme, anti-oxidation and anti-inverse protein up-regulated, and transgenic hydrogen peroxide, and carbon dioxide acid enzyme, anti-oxidation anti-inverse protein expression down-regulated, and the member protein of Glutathione-ascorbate cycle system expression up-regulated, This means that transgenic-there may be a protective mechanism to reduce oxidative stress in pressure, so as to transgenic provides the basis of potato genotypes with tolerance to low potassium.