| In this study, by referring to the regeneration system of Dendrocalamus hamiltonii, mature zygoticembryos of24species of bamboo which belong to9genera were used as explants for the research of thecallus induction and plant regeneration. Finally6species of bamboo which had stronger regenerationcapacity were selected. Meanwhile, the regeneration systems of Gigantochloa tekserah, G. brevisvagina,G. felix were optimized, and the related transgenic research was carried out. The main results are asfollows:1. Comparison of the regeneration capacity of different species of bambooThe callus induction and multiplication medium was the same as the mediun used in theregeneration of D. hamiltonii. In this medium, the callus of23species of bamboo could be induced. Thefrequencies of the callus were different, and the highest was98.34%.7species of bamboo which belongto Bambusa, Dendrocalamus, Gigantochloa and Thyrsostachys could be induced to form compact andgranular callus. The callus differentiation medium was changed by reducing the concentration ofnaphthaleneacetic acid to0.3mg·L-1. In this medium, the normal plantlets of B. polymorpha, G.brevisvagina, G. felix and G. tekserah could be successfully regenerated from callus. Meanwhile, theabnormal plantlets of D. barbatus, the albino seedlings of G. apus, and the green callus of T. siamensiswhich could not be regenerated into plantlets were obtained. The research showed that the regenerationcapacity of bamboo which belong to Bambusa, Dendrocalamus, Gigantochloa was sronger than others.The medium used in the regeneration of D. hamiltonii had certain commonability, but it was not optimalto all species of bamboo.2. Optimization of the regeneration system of G. tekserah（1）3mg·L-12,4-dichlorophenoxyacetic acid was optimal for callus induction of G. tekserah andG. felix. The result was the same as that in the regeneration of D. hamiltonii. In this medium, thefrequencies of callus of G. tekserah and G. felix were97.83%and100%respectively, the frequencies ofgranular callus of them were31.23%and43.91%respectively.（2） Adding3mg·L-1abscisic acid in the callus induction medium of G. tekserah and G.brevisvagina was not only beneficial to the induction of granular callus, but also could improve thefrequency and the process of callus differentiation. The multiplication coefficient of callus and the ratio of embryogenic callus could be improved with3mg·L-1abscisic acid. The multiplication coefficient ofcallus which was cultured in MS medium supplemented with30g·L-1sucrose,0.3mg·L-12,4-D,500mg·L-1Gln,500mg·L-1Pro,500mg·L-1CH and8g·L-1Type A agar was up to0.68.（3） The regenerated plantlets of G. felix grew well in the optimal callus differentiation mediumwhich was MS medium supplemented with30g·L-1sucrose,1mg·L-1BA,1mg·L-1kinetin KT,0.1mg·L-1NAA and3g·L-1gelrite, and the callus differentiation rate was23.33%.3. Transgenic research of bambooRNA interference vector of High Tillering and Dwarf2（HTD2） gene related to rice tillering wastransformed into the callus of G. brevisvagina, G. tekserah and D. hamiltonii byAgrobacterium-mediated transformation. Six plantlets of D. hamiltonii （including3albino plantlets）,callus with green spots and roots differentiated from callus of G. brevisvagina were obtained, and havenot been detected. |
