Using Molecular And Cytogenetic Analysis Of Safflower Varie Ties Resources And Ricehybrids Identification

This research using molecular and cytogenetic techniques analysis14varietiesof safflower resources of six provinces in China to investigate the genetic relationshipbetween the material; Rice and Sudan grass hybrid progeny were identified.The mainresults are as follows：Karyotypes of3different Carthamus tinctorius L varieties (Xinjiang Kuche,Anhui, Yunhong3#--XJ, AH, YH3#) were investigated. The number of chromosomesin Carthamus tinctorius L was calculated by the method of flame-dying protocol afterenzyme digestion of cell wall was used to prepare chromosome. Results showed thatthe number of chromosomes of XJ was24. Its Karyotype was formulated as2n=2x=24=22sm+2st which indicated Stebbins’s3B type. The number of chromosomes ofAH was24. Its Karyotype was formulated as2n=2x=24=18sm+4m+2st whichindicated Stebbins’s2B type. The number of chromosomes of YH3#was24. ItsKaryotype was formulated as2n=2x=24=10m+10sm+2st+2M which indicatedStebbins’s2B type.YH3#safflower genomic DNA probe itself on each chromosome are uniformlycovered, indicating that the genomic DNA probe for GISH analysis of theexperiments to be reliable. AH GISH results hybridization signal in AH chromosomecoverage more comprehensive, but less coverage in the7,8-chromosome. The GISHresults of the XJ on chromosome hybridization signal comprehensive coverage,whether it is in the centromere position or end position or near the centromereposition in XJ, YH3#the genomic DNA probe signal has a good coverage.Using4kinds of chloroplast DNA sequence, analysis of12safflower cultivarsin different regions in China, analysis equence GC，constructing phylogenetic treesobtained the evolution of four genesspeed relations for trnL-F sequence> thetrnS-trnfM sequence> the psbA-trnH sequence, rps16sequence. Differentgeographical safflower tree analysis showed no significant correlation of geneticdiversity and geographic differences.Preliminary analysis on the safflower genome satellite DNA usinghigh-throughput sequencing technology.20repetitions of the repeating unit arrangedto determine the part of the part of the predicted repeat sequence type Dotplotsoftware to do their own comparison, analysis of the direct repeat sequence. Most CL26repetitions.Karyotypes of Sorghum sudanense and hybrid progeny with rice wereinvestigated. Results showed that the number of chromosomes of Sorghum sudanensewas20. Its Karyotype was formulated as2n=2x=20=20m which indicated Stebbins’s2A type. The number of chromosomes of YP1was24. Its Karyotype was formulatedas2n=2x=24=14sm+10m which indicated Stebbins’s2B type. The number ofchromosomes of YP2was24. Its Karyotype was formulated as2n=2x=24=14m+10sm which indicated Stebbins’s2B type, The number of chromosomes ofYP3was24. Its Karyotype was formulated as2n=2x=24=10sm+12m+2st whichindicated Stebbins’s3A type, The number of chromosomes of YP4was24. ItsKaryotype was formulated as2n=2x=24=20m+4sm which indicated Stebbins’s3Btype, The number of chromosomes of YP5was24. Its Karyotype was formulated as2n=2x=24=18m+6sm which indicated Stebbins’s2A type,Genome in situhybridization (GISH) procedure was adopted to analyse the genomes of Sorghumsudanense and hybrid progeny with rice using the genome DNA of Sorghumsudanense as probes. The results showed that genomic DNA in hybrid progeny withrice genome coverage, Can be determinedare the true offspring.Preliminary analysis on the safflower genome satellite DNA usinghigh-throughput sequencing technology.20repetitions of the repeating unit arrangedto determine the part of the part of the predicted repeat sequence type Dotplotsoftware to do their own comparison, analysis of the direct repeat sequence. MostCL26repetitions.Rice and Sudan grass hybrid progeny identificationRice with Sudan grass hybrid progeny analysis and study, that the hybridoffspring chromosome numbers are2n=24, the same as the number of ricechromosome karyotyping. Karyotype was formulated as2n=2x=20=20m whichindicated Stebbins’s2A type. Its Karyotype was formulated as2n=2x=24=14sm+10mwhich indicated Stebbins’s2B type. SSR molecular markers RM172specific bands inSudan grass and YP3, YP4RM202Sudan grass and YP1, YP5specific bands, RM212on Sudangrass andYP2specific bands.The GISH results show that higher thesudangrass rice affinity, suitable for hybridization. SSR markers results showed thatoffspring are true hybrids.