Pathogen(Suspected) Study Of Aural Abscesses In Chrysemys Picta Picta

Special pets such as turtles are welcomed by people recently. Chrysemys pictapicta is kept as pets because of its petite body and captivity of adapt to farming. Usingthe research methods of molecular biology combined with classic traditional strainidentification method, this paper determined the species of strains that isolated from aChrysemys picta picta with aural abscesses, which were offers much theoretical basison aquaculture in the future．The symptoms of Chrysemys picta picta with aural abscesses were swellings onone or both sides of the head, and inflammation or abscess of the tympanic cavity.Although some reports appears to be the result of several predisposing factors, theexact cause of aural abscesses cannot be definitively stated. Three bacteria strainsnamed CPP120505, CPP120505-1and CPP120505-2were isolated from the infectedear of Chrysemys picta picta with aural abscesses. The artificial infection adopted byimmersed and injection, and the concentration of the strains were3×10~8CFU/mL. TheArtificial infection experiments include intraperitoneal injection and soak infection,and the concentration of the strains were3×10~8CFU/mL, which both proved that thethree strains were pathogens and had strong pathogenicity to Trachemys scripta, whosefatality rate were80%and70%,50%and40%，70%and60%respectively. The reasonis unclear that not appear the symptom of ear infections, so the cause of turtle earabsecesses need to be further research.According to the morphological, physiological and biochemical characteristics,the strain CPP120505was preliminary identified as Morganella morganii and thepossibility is99.99%. The strain CPP120505-1was preliminary identified asAeromonas hydrophila and the possibility is98.56%. The strain CPP120505-2waspreliminary identified as Aeromonas sobria and the possibility is98.96%. Thedominant strain was isolated from the focus of the artificial infection. The strain CPP120505was further verified by the molecular biological level through16S rDNAgene sequence determination, because compared with the16s rDNA gene sequences ofMorganella morganii in GenBank database, the strain CPP120505located in thephylogenetic tree has the highest homology(98.6%-99.2%) with it.The K-B drug sensitivity test using18antimicrobial agents showed that the threestrains have the similar results that they all susceptible to9agents includinggentamicin, tobramycin, erythromycin, streptomycin, kanamycin, ofloxacin,ceftazidime, piperacillin, chloromycetin, and resistance to5agents including cefazolin,tetracycline, amoxicillin, rifampicin, ampicillin at the same time, which may beassociated using various kinds of antibiotics on aquaculture. This research fills theblank of pathogens field, which provide basic data and theoretical basis for theprevention and treatment of the turtle with ear abscesses.