Oxidative Damage Of Botrytis Cinerea Spore Caused By Oligochitosan

Fruit rot caused by Botrytis cinerea is one of the factors restrict the development of fruitindustry in China. Chemical pesticides are widely used after fruit postharvest, but it willpollute the ecological environment, enhance disease and insect resistance and also harmfulto human health. Therefore, it is important to research and develop non-toxic, non-pollutinggreen bactericide to protect the ecological environment, reduce pesticide residues in food,ensure human health. Oligosaccharide as a kind of natural positively charged molecules hasbroad-spectrum antibacterial, regulating plant growth and development, improve the diseaseresistance and inhibit the action of the disease occurs, is known as a good applicationprospect of biological pesticide, but its bacteriostatic mechanism is lack of definite cognition.ROS as a universal signal molecule in organisms, can be involved in normal physiologicalreaction, also be harmful to the cells. This experiment explore the ROS level and oxidativedamage after joining the oligosaccharide, aim at reveal its inner mechanism with inhibition ofspore germination.1.With the setting up of the group of blank,0.05%and0.5%, we can see that as theincrease of oligosaccharide and the extension of processing time, Botrytis cinerea showedlower germination rate, higher H₂O₂content, deepen lipid peroxidation degree, deepenprotein damage, DNA damage and apoptosis level increases.2.With the addition of H₂O₂, H₂O₂within Botrytis cinerea is added and increased thelipid peroxidation, protein oxidative damage, DNA oxidative damage, apoptosis, and evenresulted in the death of cells.3.With the addition of Vc, inhibition of H₂O₂,lipid peroxidation, protein oxidation injury,oxidative DNA damage and apoptosis generated.4.With the addition of CAT, results show a greater degree of inhibition of the H₂O₂generated, lipid peroxidation, protein oxidation injury, oxidative DNA damage and apoptosis.In conclusion, research results show that the oligosaccharide may induce the increase ofROS, high concentrations of ROS induce plasma membrane lipid peroxide damage, protein carbonylation and nitro damage, DNA damage, apoptosis and losing of function.