Endophytic Fungus Schizophyllum Commune HC-01from Hemerocallis Lilio-asphodelus Produces Hemerocallin

Hemerocallis lilio-asphodelus is a kind of poisonous plant of Hemerocallis Liliaceae, theroots of which contain hemerocallin, asparagine, colchicine and other toxic ingredients. Fedby animals, it causes damage of systemic acute poisoning to the central nervous system. Theclinical manifestations are mydriasis, blindness, paralysis, cystoparalysis and so on. Atpresent, the study of toxic Hemerocallis plants has made some progress: For one, the maintoxic component in Hemerocallis lilio-asphodelus is Hemerocallin; For another, it was studiedthat controlling the content of Hemerocallin can reduce the toxicity of Hemerocallis plant.The object of this study is the Hemerocallis lilio-asphodelus, from which one strain ofendophytic fungi was isolated and obtained.It lays a good foundation for the research ofHemerocallin’s poisoning mechanism.The study was to explore whether the hemerocallin-secret fungal endophyte exist in theroot of Hemerocallis lilio-asphodelus. Two strains of endophytic fungi were isolated andobtained from the Hemerocallis lilio-asphodelus. The two isolates were detected by thin-layerchromatography and UV spectrophotometry after culture in potato dextrose agar (PDA) for20days. Result showed that a strain of endophytic fungus produced the hemerocallin, HC-01,was isolated and obtained from Hemerocallis lilio-asphodelus, and hemerocallin yield is359.88μg/g. Morphological evidence and analysis of the ITS sequences indicated that theendophyte was closely related to Zalerion varium. The hemerocallin-producing fungalendophyte exist in the root of Hemerocallis lilio-asphodelus.