Research On The Impact Factors Of Culture Of In Vitro Female Nucleus And Plant Regeneration In Squash (Cucurbita Pepo L.)

This experiment used5different varieties as experimental material,studied the impactfactors of culture of in vitro female nucleus and plant regeneration.Mainly discussed theinfluence of embryoid culture from plant genotype、concentration of TDZ、time of heatshocking、 cultivation season,and screened appropriate culture conditions of embryoidculture.And preliminarily explored the impact factors of transplantation survival rate fromplant morphology、acclimation temperature and the way of hardening-seedling to acquire theculture system which is favorable for culture of in vitro female nucleus.The results of this experiment show that:1.Genotype has has a major influence on embryoid induction of culture of in vitro femalenucleus,there are relative difference between the time of embryoid occurring and embryoidinductivity.In the five materials,the embryoid inductivity of XH11-24is highest,and its timeof embryoid occurring is shortest, respectively are18.90%and33d.2.Adding TDZ hormone to culture medium has a certain acceleration on embryoidinduction.In the five concentrations, adding0.06mg/L TDZ to medium,the inductive effect ismore better，the embryoid inductivity is highest,is17.17%.3.Under the same heat-shock temperature（35℃）,different heat-shock time has differentinfluence on embryoid induction.In the five time,120h has most ovules turning green andhighest embryoid inductivity,respectively are86.76%and18.57%.4.In different seasons,the embryoid inductivity is different.X11-24and X11-55,theirembryoid inductivity of autumn materials are higher than spring materials,respectively above5.40%and3.43%.5.There is obvious difference on survival rate of transplantation between differentmorphology of regenerated plantlets.When they have5-6true leaves,the survival rate oftransplantation is highest,is87.61%.6.The survival rate of transplantation of regenerated plantlets have difference when theacclimation temperature is different.In the three temperature gradient, when acclimationtemperature is day temperature21℃-27℃、night temperature10℃-20℃,the survival rate oftransplantation is highest, is88.27%. 7.Different ways of hardening-seedling have different influences on survival rate oftransplantation.In the three different ways,firstly take illumination training and then takehumidity training,the survival rate of transplantation is highest,is90.47%.