| The objective of the present study was to investigate the influence of pre-chillingtreatment on the drought and salt resistance of Perennial Ryegrass, and to improve foragegrasses yield in drought and saline soil.（1）The perennial ryegrass （Lolium perenne L.） seeds were treated at3-5℃for1,35d and not treated at3-5℃as the control. The germination capacity of all groups immersedin PEG6000（0%,5%,10%,15%,20%,25%）were measures. It was shown that when thePEG≥15%, the germination index and vigor index of perennial ryegrass seeds treated inpre-chilling were significantly increased in5d compared with control group. There is nodifference between1d and3d for the germination index and vigor index of perennialryegrass seeds treated in pre-chilling. Pre-chilling hasn’t affected on root growth rate and rootactivity（P﹥0.05）.With the mass fraction of PEG6000increasing, the contents of proline andsoluble sugar had not changed regularly. When the mass fraction of PEG reached≥15%,pre-chilling reduced significantly the degree of membrane lipid peroxidation（2）After the perennial ryegrass （Lolium perenne L.） seeds were treated at3-5℃for3,5, and7d, and not treated at3-5℃as the control, the germination capacity of all groupsimmersed in NaCl（0%,0.3%,0.6%,0.9%,1.2%） were examined. When germination wastested, the content of MDA, the content of proline, the root vigor and the content of nitricoxide （NO） were examined. It was shown that when the seeds were exposed to subsequentNaCl stress, the germination index and vitality index of perennial ryegrass seeds treated inpre-chilling for3d or5d significantly increased compared with control group. Meanwhileappropriate pre-chilling reduced significantly the degree of membrane lipid peroxidation andincreased the root vigor. Pre-chilling treatments markedly improved the content of NO.Pre-chilling treatment for3-5d could improve the salt resistance of Perennial Ryegrass seedsduring the germination, NO might have regulatory effect on plants’ growth. |
PDF Full Text Request