Study On Anti-membrane Lipid Peroxide Ability And Genetic Stability Of Regeneration Plant After Droplet Vitrification Cryopreservation Of Huaiqing Chrysanthemum

The excellent variety of Huai chrysanthemum, Xiaohuangju had been used as experimental materialsin this study. Based on the droplet vitrification cryopreservation system of the former establishment, wehad done a system research about the anti-membrane lipid peroxide ability and genetic stability of theregeneration plantlet, and gotten the following results:1. Droplet vitrification cryopreservation had improved the anti-membrane lipid peroxide abilityof its regeneration plant.Droplet vitrification cryopreservation had improved the low temperature tolerance of its regenerationplant and the anti-membrane lipid peroxide ability. But the advantage of anti-membrane lipid peroxide ofthe anti-membrane lipid peroxide of the regeneration plant would gradually weaken from third to eighthsubculture.（1） Compared with normal temperature controlled plants （named as CK）, during low temperaturetreatment, the leaf wilting and frostbite phenomenon of regeneration plant from droplet vitrificationcryopreservatio （named as RPDC） was delaied, and its frostbite was obviously lighter. SOD and PODspecific activities of its leaves were higher than CK and strengthened in the later stress stage. At the sametime, the absolute value of the content of O2·-was decreased.（2） Compared with normal temperature controlled plants, in the process of subculture, the SOD、APX、CAT and GR specific activities in the leaves of RPDC were improved to some extend from3to5generations, and POD enzyme specific activity had no significant difference, the content of MDA, H₂O₂and O2·-from3to5generations were lower to some extend. The band brightness of SOD isozyme hadobvious differences from third to fifth subculture generation, and no obvious difference from sixth to eighthsubculture generation. And POD isozyme bands have no obvious differences in all six subculturegenerations.2. The genetic stability of RPDC had no change.（1） RPDC kept up the genetic stability on DNA molecular level.The two ISSR primers were selectedfrom75ISSR primers to determine the genetic stability of RPDC. There were no difference bands among 19ISSR fragments detected. That indicates: RPDC keep up genetic stability on DNA molecular level.（2） RPDC kept up the genetic stability on agronomic characters and physiological. In the process ofgrowth in the field, RPDC and CK from July to October have no significant difference in plant height,crown width, soluble sugar content, soluble protein content, chlorophyll content, SOD and POD enzymespecific activities. But the flowering stage of RPDC was be delayed, and each stage were be3⁵dayslater than CK. It showed that RPDC could keep up the stability of agronomic characters and physiological.（3） RPDC kept up the genetic stability on yield and quality. We analyzed two years production ofRPDC and CK in two test areas, and found that the flower and tiller numbers per capita of RPDC weremore than CK, and the fresh weight per plant, dry weight per plant, fresh weight per mu and single flowersize were slightly lower than their control, but to each output indexs, there were no significant differencesbetween RPDC and CK. The chlorogenic acid content of flowers had also no significant difference betweenthem. It showed that RPDC could keep up the stability of yield and quality.（4） RPDC kept up the genetic stability on isozyme spectrum. In the process of growth in the field,RPDC and CK from July to October had no significant difference in SOD and POD isozyme.