Study On The Tissue Culture And Rapid Propagation System Of Aronia Melanocarpa Elliot

Aronia melanocarpa Elliot is one of the precious tree species with high drought-resistance and cold-resistance which fruit is full of variable nutrition and pharmacy substance and has eating, pharmacy, gardening and ecological values. The seed belongs to deep-sleeping type and it cuttage is difficult to growth. In order to establish the tissue culture and rapid propagation system of Aronia melanocarpa Elliot, stems with lateral buds were used as explants to explore the effects of proliferation. Meanwhile the vitrified shoots were used as explants to explore the suitable reversal conditions. We studied the shoot characteristics by means of preparing paraffin section and measuring physiological and biochemical criterion. Furthermore, rooting conditions and transplantation media were screened and optimized. All of the above provided a theoretical foundation for growth mechanism and scale production for Aronia melanocarpa Elliot.The factors on proliferation of Aronia melanocarpa E. were studied. The results showed that MS was the suitable basic medium. The hormone treatment of BA0.5mg·L-1, NAA0.3mg-L"1was better for growth and proliferation, resulting in fresh weight and dry weight reaching the maximum respectively with a proliferation coefficient of6.4. The stem tip of2.0±0.1cm was the best part for explant, leading the proliferation up to7.2cm and the shoots growth well with less vitrifid shoots.Vitrifid shoots were used as test materials in reversion experiment to explore suitable conditions for reversing to normal shoots. The experimental results indicated that the BA concentration was0.3mg-L"1can promote proliferation and effectively reduce the vitrification phenomenon. When using glass bottles with filtration as culture flasks, the proliferation coefficient reached to4.8with spread leaves, while the vitrification rate reduced to12.3%. Upwards of2400lux light intensity at the same time, effectively making the vitrifid shoot reverse to normal ones, the vitrification phenomenon was almost vanished.Observation of morphological microstructure and determination of physiological and biochemical criterion showed that stomas of lower epicuticle thickness of Aronia melanocarpa Elliot were small and dense, palisade tissue consisted of2-3layers of cells, spongy tissue and cell gap were not well-developed, there were abundant vessels and sieve tubes in stem. In contrast, main veins of vitrified shoots were irregular and messy and not obvious. Stomas opened circularly, Differentiation between the palisade tissue and spongy tissue was not obvious. The stem was swelling and thick with the parenchyma cells of pith had been broken. Water content, relative water content and soluble sugar content of vitrified shoots were significantly higher than normal, but chlorophyll content, soluble protein content and superoxide dismutase activity were lower than normal, the activity of peroxidase was about40%lower than normal shoots.We researched appropriate rooting condition and transplant domestication for Aronia melanocarpa E. The results indicated that:for plantlets cultured with1/2MS and0.6mg·L-1IB A, its root length can be as long as3.6cm, the average root quantity was4.4with stretch leaves, medium leaf size, and the number of leaves were5-7, being beneficial to transplanting. The suitable transplanting medium was vermiculite:pearlite=2:1, resulting in survival rate reaching to98.7%, when transplanted to outdoor gradually, they can grow healthily and winter successfully.