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Inheritance Of Tuberculate Trait, Variety Trials And Fingerprinting Construction In Cucumber ’L(?)dao5’

Posted on:2014-05-12Degree:MasterType:Thesis
Country:ChinaCandidate:D DingFull Text:PDF
GTID:2253330401490774Subject:Gardening
Abstract/Summary:PDF Full Text Request
On the basis of the study of the hereditary laws of the size of the Ji Dongautumn cucumber`s fruit tumor, the author made15hybrid combinations with2large papilloma cultivars as core parents and the main cultivars (Lǜ feng wang andLǜ guan) as control cultivars, and obtained ‘Lǜdao5’ preparatory which is theexcellent target trait crosse. Furthermore, to further promote the update of the JiDong autumn cucumber varieties, the author built its fingerprint with RAPDtechnology. The main results are as follows:1. The size of the Ji Dong autumn cucumber’s fruit tumor is controlled by onepair of complete dominant gene and the bigger fruit tumor is dominant inheritance.2.15autumn cucumber crosses preliminary comparison test,Further varietytests and production test showed that ‘Lǜdao5’ has multiple targets of quality,disease resistance, stress tolerance, high yield.‘Lǜdao5’ products Guagua about12cm long, commodity weight about147g, melon-shaped short cylindrical,cucumber’s color uniform light green greenish, large papilloma, white thorn, melonshort dark green, thick flesh light green, taste sweet, sour fragrance, its commodityof melon soluble sugar content, soluble solids content, sugar/acid, vitamin Ccontent which were significantly higher than the two control varieties Lǜfengwangand Lǜguan, titratable acid content with the control varieties of Lǜguan nosignificant difference was significantly lower than the control varieties Lǜfengwang;cucumber powdery mildew disease resistance was significantly higher than the twocontrol varieties Lǜfengwang and Lǜguan. Plant growth medium, the main vineresults-based, weak branches, leaves smaller, compact plant cold tolerance.‘Lǜdao5’ yield is morn than the control varieties Lǜfengwang an increase of10.5%~24.3%,is morn than the control varieties Lǜguan an increase of15.6%~18.5%, is morn thanthe control varieties Guifeicui an increase of17.8%.‘Lǜdao5’ has multiple targetsof quality, disease resistance, stress tolerance, high yield and other traits. Best suitedfor spring and autumn and winter stubble solar greenhouse and plastic greenhouses in winter and spring cultivation Jidong.3. The fingerprinting of Cucumber ‘Lǜdao5’ were established compared withthe similar cucumber types of Lǜfengwang、Lǜguan and Guifeicui using RAPDmolecular markers.Five markers, AJ10、 E18、 M18、 S331and S484, werescreened from120RAPD primers,which generated five polymorphic lociFoursimilar cucumber cultivars would be identified by the fingerprinting results withhigh resolution(1/135000).
Keywords/Search Tags:autumn cucumber, papilloma genetic, quality, resistance, yield, cold tolerance, fingerprinting
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