Genetic Characteristics Of Newcastle Disease Virus In Shanxi

To explore the epidemiological Status of Newcastle disease virus (NDV) in chicken, five NDV strains isolated from outbreaks in chicken flocks in Shanxi Province during the years2011-2012were analyzed for the molecular epidemiology in the present studies. According to the F gene sequence of NDV strains in GenBank (FJ969394), primer was designed and synthesized. The F genes of isolated strains were amplificated with specific primers by RT-PCR, then cloned into PMD19-simple T vectors, certified by PCR and sequenced. By using DNAstar7.1software, Comparison has been made on the nucleotides of the gene F and their deduced amino acid sequence with represent NDV strains. The genotypes of the five strains have been established by Phylogenetic tree.By analyzed the sequence of the F gene, four strains belonged to genotype Ⅱ, which homology of nucleotide sequence were up to99.2%-99.4%and amino acid sequence were100%compared with LaSota strain. the cleavage site was composed of112G-R-Q-G-R-L117, which revealed that these4strains were attenuated strains. The homology of nucleotide and amino acid sequence of the other strain were95.6%and97.5%, shared with JS/5/01/Go strain which belonged to genotype VIId. the cleavage site of this isolated strain was composed of112R-R-Q-K-R-F117and have structure of K101andV121, which revealed that it was velogenic strain.