Swine Fever Antigen And Antibody Detection Methods And Its Significance

In order to study the relevance of clinical diagnosis and disease prevention and control of classical swine fever antigen and antibody detection, This experiment used abortion sows、abortion stillbirth fetus and sudden death nursery pigs in the large farms to detect swine fever antigen detection (ie, utilize the method of RT-PCR detecting the presence of classical swine fever virus infection); choose2litters (denoted as A and B)18healthy piglets, born the same day, Collecting blood respectively on1-day-old(ie newborn),8-day-old,15-day-old,22-day-old test classical swine fever antibody levels, and detected the corresponding sow swine fever antibody levels at the corresponding time points, the main results were obtained as follows:1, the detection of abortion sow blood were20parts, wherein the detection CSFV positive result has two parts, the positive rate was10%. Detected miscarriage stillbirth fetus epidemic materials45parts and the results were negative.2, the detection of sudden death conservation swine disease materials were35parts, which included classical swine fever virus positive results3parts, the positive rate was8.6%.3Experimental results show that the detection of piglet swine fever antibodies on1-day-old (newborn) piglets the S/P values were0(that means no swine fever antibody), Antibody levels reached the highest peak in the experiment of1.72±0.29(A)0.84±0.16(B) on8-day-old, Compared with8-day-old, the antibody levels on15-day-old were decreased by13.4%(A)and33.3%(B),the antibody levels on22-day-old were decreased by26.8%(A)and52.4%(B); Compared with15-day-old, the antibody levels on22-day-old reduced by15.4%(A)and28.6%(B), the above changes have significant difference (P<0.05); at the same time, the piglets’ antibody level was significantly related to their sows CSFV antibody level; A litters’antibody positive rates were100%in the whole process, the antibody level of sow A has been positive, and continued in a rising state; B litters on8-day-old the antibody positive rate was up to90%, to15-day-old the positive rate was dropped to50%, and to22-day-old, the positive rate was only30%, the antibody levels of sow B were on the decline, the antibody level of sow B in22-day-old was negative (the S/P value was less than0.5). Summing up the results of the present experimental conditions, the swine fever antigen overall detection rate was5%, and the sows’ swine fever antibody levels decided the antibody levels of their respective piglets.