| Birch (Betual platyphylla) is a species of northern China with high economic value. Now the sutdy on Betual platyphylla has been accomplished good progress, but the flowering developmental mechanism is not very in-depth. To learn more about the background of birch flowering gene expression, we use a new generation of high-throughput sequencing Solexa and bioinformaties analysis teehnology to study the white birch male and female inflorescences transcriptome. It can help us identify candidate genes related flower development, and to provide some valuable information about follow-up functional genes research and molecular breeding practices. The birch SEP1gene cloning and expression analysis were carried out by RACE, and its biological function was studied through transgenic Arabidopsis. Our results are as follows:(I) We generated107,102unigenes, yielding approximately4.3Gb of sequence data, a total of25,027,337readings, including female inflorescence of49,516and male inflorescence of57,586. The number of unigenes overlkb in length is32,706, accounting for30.5%of the total number of unigene libraries.(2) Based on RPKM method, we gained2671significantly differentially expressed unigenes in brich male and female inflorescence transcriptome. Of that1985unigenes match to Gene Ontology database,971unigenes match to COG database,716unigenes match to KEGG pathway database.(3) A total of8163SSR loci were obtained by SSR analysis and a total of10,845SNP loci were found by SNPs detection.(4) Bp1SEP1gene was isolated from Betula platyphylla by RT-PCR and RACE technology.The full length of Bp1SEP1cDNA is928bp containing732bp open reading frame, which encodes243amino acids. QRT-PCR showed that Bp1SEP1gene is expressed in all tissues of Betula platyphylla, but at higher expression levels in the reproductive organs.(5) Transgenic Arabidopsis displayed that Bp1SEP1gene not only be able to regulate the development of the floral organs, but also may regulate lateral organ development. |
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