Expression And Functional Analysis Of CD36Gene In Zebrafish

CD36is a transmembrane glycoprotein and one of class B scavenger receptors. It is foundin many mammalian tissues and cells, and participates in a variety of physiologicaland pathological processes, such as immune regulation, fatty foods taste experience,cell adhesion, angiogenesis and obesity. Current researches focuse on mammals, but itis still very weak in fish. The zebrafish is an important fish model organisms. Recently,the distribution of the the zebrafish CD36gene organization, spatial and temporalexpression patterns during embryonic development and its role in the immunepreliminary study. In this paper, we do zebrafish CD36gene prokaryotic expression invitro based on our previous study,.Part of zebrafish CD36gene was obtained by PCR,eccoding395amino acids with apredicted molecular mass of approximately46.8kDa.It is1185bp long.we constructedthe recombinant prokaryotic expression vector with pET-28a. We find most recombinantCD36protein was in inclusion bodies through analysis. The refolded protein can inhibit thegrowth of Gram-negative bacteria E. coli and Gram-positive bacteria S. aureus. TheFITC-labeled recombinant protein can bind to Gram-negative bacteria E. coli andGram-positive bacteria S. aureus, suggesting that the recombinant proteinbacteriostatic mechanism may be that the recombinant protein can combinate with thebacterial surface-active substance. CD36domains mainly are located between93-183amino acid, so we designed a pair of targetting sites,and constructed two TALENexpression vectos successfully with NheI,HindIII and SpeI, which provides a foundationfor the study of CD36.