Efficacy And Tolerance Of Montmorillonite As Mycotoxin Adsorbent In The Hybrid Sturgeon’s Feed

Mycotoxin adsorbent is one of cost-effective way of detoxification for feed producer. However,the efficacytolerence test were not integretedly conducted in aquaculture animal feed yet. In presentstudy, we selected hybrid sturgeon (Acipenser schrenckii×A.baeri) as target animal todetermine the effect of domestic montmorillonite adsorbent on growth, haematology, immunityresponse and liver/intestinal health by two experiments. The details are shown as follows:(1) Tolerance test for montmorillonite mycotoxin adsorbent in the hybrid sturgeon feed:choosing hybrid sturgeon as experimental animals whose initial average weight are45.1+0.12g anddomestic montmorillonite mycotoxin adsorbent as subject content, this experiment designed fivelevels, basal feed contained fish meal, soybean meal and powder as the main source of protein,soybean oil, fish oil and lecithin oil as fat source, fresh powder and flour as carbohydrate source, withAFB1<10μg/kg. On basis of these, adding0%,0.5%,0.5%,2.5%and5.0%of montmorillonite,respectively named H0, H0.5, H1.0, H2.5and H5.0.1.0%is the the highest doses recommended forenterprises.With aflatoxin B1in feed <10.00ug/kg, adding0.5%montmorillonite could significantlylower the growth performance of the hybrid sturgeon; Add1.0%montmorillonite had no significanteffects on all the indicators; Adding2.5%~5.0%montmorillonite could harm the the intestinal tractand liver histology and weak the growth performance, liver function, immunity and anti-oxidationfunction significantly. Montmorillonite as mycotoxin adsorbent is suggested adding between0.5%and2.5%into fish feed.(2) Research for the effect of Montmorillonite on afatoxin B1in juvenile hybrid sturgeon feedand the tolerance of hybrid sturgeon to the Montmorillonite: choosing hybrid sturgeon asexperimental animals whose initial average weight are11.5+0.01g and domestic montmorillonitemycotoxin adsorbent as subject content. On basis of these, adding0%,0%,1.0%and10.0%ofmontmorillonite, respectively named D1, D2, D3and D4,and adding40μg/kg AFB1,which is thehalf lethal dose to this fish, into D2,D3and D4.1.0%is the the highest doses recommended forenterprises.The experiment last8weeks,aiming to study the effect of montmorillonite on afatoxinB1in juvenile hybrid sturgeon feed and the tolerability of juvenile hybrid sturgeon to themontmorillonite. With aflatoxin B1in feed=40.00ug/kg, adding1.0%montmorillonite could notsignificantly improve the blood biochemistry, nonspecific immunity and oxidation resistance.Adding10.0%of montmorillonite could significantly harm the the intestinal tract and liver histology andweak the growth performance, liver function, immunity and anti-oxidation function,especiallyNBT,NO and SOD. Montmorillonite as mycotoxin adsorbent is not suggested adding into juvenilehybrid sturgeon (11.5+0.01g) feed.