Phylogeny And Genetic Diversity In Eysarcoris

Eysacoris is belonged to Pentatominae, Pentatomidae, Heteroptera, Hemiptera. They are important agricultural insect pests that mainly eat plants’ juices of vegetative organs and reproductive organs. In recent years, the research of Pentatomoidea mainly concentrated in the morphology.In this study, the morphologies of testis and chromosome, phylogenetic relationships and molecular genetic diversity of several Eysacoris were examined using COI,16S rRNA and Cytb sequences for the last two parts. The main contents and conclusions are as follows:The first part, the shape, color, size, position description of testis of Eysacoris fallax and Eysacoris aeneus were observed and described. Results showed that:in Eysacoris, the variation of morphology, size, position, distance, area, the color of testis. in interspecific is not obvious and it can not be used for classification and phylogenetic analysis. While the connecting way, length, diameter of spermaduct can be used in in interspecific identification and evolution. The number, size, morphology, behavior and sex determination mechanism of chromosome characteristics of E.fallax were observied and described and the number and size of the chromosome were verified suitable interspecific classification features.The second part, phylogenetic studies of several species of Eysacoris were analysed based on molecular sequences. The aligned1641bp Hox genes(COI,16S rRNA and Cytb) were chose as molecular markers. Bayesian Inference (BI), Maximum parsimony (MP) and Maximum likelihood (ML) methods were used to generate the phylogenetic trees. The topologies of three trees were basically the same and E. aeneus was treated as the base branch.The third part, the analyses of nucleotide diversity and haplotype diversity analysis showed that E.aeneus has the highest diversity; TCSnetwork chart did not show obviously spread path, perhaps special living environment requirements limit the spread of the population and the spread route of choice.