The Research About Morphological Characteristics And Physiological Biochemical Character Of Different "Sanghuang"

The research contains two parts about "sanghuang", the materials of first group were from different places, including ten strains and six fruit bodies. The frist step we compared their growth speed, the percentage of active compounds in fermentation product between ten different "sanghuang" strains which kept in our lab, and the results could provided biology index to assess strain character. Then we compared the valuable composition content in five wild fruit bodies with different harest time and an artificial one, for quality evaluation and optimal harest time decision. The fruit bodies of second group were totally from TongHua city, JiLin province, which were all used as "sanghuang" in folk, but their scientific names and species are unknown. In order to identify these samples and assess their medicinal value, we primarily compared their appearance morphology to lay the foundation of further identification, then make a comparison of their effective components content, so that we could provide a guidance for folk medicine and a first standard for excellent strains selection.Ten strains were respectively from AnHui province, Jilin province, Hebei province, Heilongjiang province, Liaoning province and the Institute of Medicinal Plant. Growth speed, extracellular Polysaccharide and extracellular flavone were inestigated as quality standards. The result showed that strain SA4grew fastest, with a speed of3.48mm/d; SA7has the highest amount of extracellular flavone(1.8383g/l) and the highest biomass in its fermentation product (0.4418g/l); strain003has the highest amount of extracellular Polysaccharide, which was0.4803g/l. These results can be regarded as guide references for data when applied in practice.Quality assessment and optimal harvesting time decision were analyzed by comparing cultivated and wild fruit bodies with different growth years old. To explore its optimal harvesting time, the content of extracellular Polysaccharide, Total flavonoid and total triterpenoids were inestigated including in five cultivated fruit bodies with one year old,2-year-old,3-year-old,4-year-old,5-year-old, and a wild one. The results show that a2-year-old cultivated fruit body produced the highest extracellular Polysaccharide, Total flavonoid and total triterpenoids of all, it is2.216mg/g,190.067mg/g,0.6045mg/g, respectively, while the wild one is1.575mg/g,54.622mg/g,0.322mg/g. It is concluded that two years old was the best harvesting time based on the result.We also discussed the current using situation of "sanghuang" in folk and the simple method to distinguish "sanghuang" from other mushrooms."sanghuang" is a precious medicinal mushroom, but there are many other mushrooms have similar appearance with "sanghuang", it is difficult for folk to identify. Up to now, there are very few relative reports about the appearance comparison of different fruit bodies, in order to compare their appearance, In this study we collected27fruit bodies of8different epiphytes from Tonghua city, Jilin province. From the result,indicated that there are more than one "sanghuang" grow on a epiphyte, and different epiphytes have similar fruit bodies parasites on them. We can connect the appearance difference with morphological identification and molecular identification, so provide a easier way as guidance for folk medicine, meanwhile we can lay a foundation for further study of.excellent strains selection.On the basis of appearance comparison, we detected different "sanghuang" samples’ active ingredients, extracellular Polysaccharide, total flavonoid and total triterpenoids, The measurement results can provideuseful data for justifying their medicinal value.