Effects Of Supplementing With Nicotinamide And Disodium Fumaric Acid On The Digestion And Metabolism Of Sheep

The objectives of this experiment were to study the effects of supplementing with nicotinamide, ordisodium fumaric acid, or nicotinamide+disodium fumaric acid on rumen metabolism, digestion andmetabolism of sheep.Experiment Ⅰ: Four healthy Chinese merino sheep(Xinjiang Type,)with similar age and body weightwere selected and designed as4×4Latin squares．Setting four treatments, such as do not add, addingnicotinamide130mg/kg.Dry matter, adding disodium fumaric acid10mg/kg.Dry matter,addingnicotinamide130mg/kg+disodium fumaric acid10g(as DM basis)d-1.sheep-1.period-1. The resultsshowed that, there was significant reduce the number of rumen protozoa, decrease the NH3-N concentrationof sheep on added disodium fumaric acid or nicotinamide+disodium fumaric acid group, and there was nosignificant effects of the number of rumen protozoa, and NH3-N concentration on added nicotinamidegroup. The content of NAD(P), NAD(P)H in rumen microorganisms of sheep were obviously increased onadding disodium fumaric acid or nicotinamide+disodium fumaric acid, but there is no significant effects ofNAD(P)H/NAD(P) ratio in rumen microorganisms. While added nicotinamide or nicotinamide+disodiumfumaric acid, the duration time of relatively low-level of NAD(P)H/NAD(P) ratio and NAD(P)H contentsin rumen microorganisms was extended. Reducing sugar concentration in the ruminal fluid wassignificantly increased when nicotinamide or disodium fumaric acid or nicotinamide+disodium fumaricacid was added.Experiment II: Twenty healthy Suffolk, with body weight of43.12±2.46and similar age wereselected, and divided into4periods, each group5sheep. The effects of adding nicotinamide, disodiumfumaric acid and adding the two elements at the same time to forage-type diet on the whole metabolism ofsheep were studied. The results showed that, compared with control group,the daily body weight gain ofnicotinamide group,disodium fumaric acid group and or both of them group were increased29.36%、32.11%、29.36%, respectively but the difference is not significant(P<0.05); Feed intake of dry matter,organic matter, crude protein, NDF, ADF, calcium and phosphorus of sheep on nicotinamide group wasincreased5.20%(P<0.05),4.87%(P<0.05),3.29%(P<0.05),6.38%(P<0.01),6.67%(P<0.05),4.74%(P<0.05),4.43%(P<0.05), digestive amount of dry matter, organic matter, crude protein,NDF,ADF,calcium andphosphorus of the disodium fumaric acid group was increased15.94%(P<0.05),11.47%(P<0.05),14.16%(P<0.05),122.25%(P<0.01),130.77%(P<0.05),40.15%(P>0.05),55.04%(P<0.01)respectively, andthe digestibility of dry matter, organic matter, crude protein,NDF,ADF,calcium and phosphorus on disodium fumaric acid group was increased11.37%(P<0.05),10.02%(P<0.05),11.45%(P<0.05),110.14%(P<0.01),117.67%(P<0.05),34.72%(P>0.05),50.33%(P<0.01),respectively; digestive amountand the digestibility of NDF and phosphorus of nicotinamide+disodium fumaric acid group was increased112.91%(P<0.01),52.52%(P<0.01),108.05%(P<0.01),50.61%(P<0.01） respectively. The differences indaily body weight gain, dry matter intake, digestibility and metabolic rate between each adding group werenot significant(P>0.05).This study showed that adding nicotinamide or disodium fumaric acid or both of them to diets couldimprove the ratio NAD(P)H/NAD(P) of rumen protozoa, increase or extend the vitality of the ruminalbacterium, improve the metabolic activity of the ruminal microbial and thus raise the nutrient intake,nutrient digestion and nutrient digestibility and metabolic rate, and improve the nutrition supply of sheep.