Genetic Material Analysis Of Drought Resistance Different Genotypes Banana

Banana (Musa paradisiacal L.) belongs to Musa of Musaceae. It is one of the most important tropical fruit in the world, while drought injury affected the production and development of the banana industry. Almost all modern edible parthenocarpic bananas come from two wild species-M. acuminate (A genome) and M. balbisiana (B genome).(Simmonds,1995). Our previous studies showed that drought resistance physiological characteristics of banana, banana genome types is not the drought resistance of determinant, but there is a tendency to show that most of the species containing the more B genome, the stronger drought resistance, the more A genome, the worse drought resistance, and the tetraploid drought resistance is stronger than the diploid and triploid. However, the cause of different genome types of banana physiological characteristics of drought resistance genetic material causes still need further research. In this experiment, the different genome types of banana germplasm, the karyotype analysis, flow cytometry, ISSR, SRAP, AFLP, MSAP molecular markers, cytological, molecular biology from two different levels of different genome types of banana drought resistance genetic material characteristics, on the basis of comprehensive analysis of drought, banana physiological characteristics and genetic characteristics, designed for banana breeding for drought resistance and drought resistant cultivation provide theoretical basis.(1) Banana karyotype analysisBrazil banana (AAA),9different genotypes of somatic cells of11banana chromosomal karyotype analysis. The results found that in Bawangling of wild banana, FHIA-03on chromosome2and Lingshui wild banana, CRBP-39on chromosome10with1pairs of satellites; FHIA-17on chromosome7with1pairs of satellite chromosomes; as well as Baihualing wild banana,1on chromosome3and chromosome85, TMBx5295-1body with2pairs of satellites, and Brazil banana, red bananas, Pisang Ceylan, Dongguan in the plantain found no satellited chromosomes.(2) Identification of banana FCM ploidy By using the known times of Lingshui wild banana (AA) as reference,9different genome types of28bananas, FCM ploidy identification. Studies have shown that:the identification results is divided into four categories, including Red Dwarf banana (AAA), AACV Rose (AA), the Siheung BB (BB), FHIA-18(AAAB), Cachaco (ABB) inconsistent and Simmonds morphological classification, belonging to different ploidy types, Red banana (AAA) also appeared2times the peak body, its genetic material needs to be further studied.(3) Banana ISSR, SRAP and AFLP analysis Three kinds of tag technology to analyze the genetic polymorphism of the28banana germplasm, the results are basically the same.28germplasm, Hong teeth banana (AAA) type, the dendrogram shows the genetic little difference, containing no obvious pattern of the B sets of genome germplasm more dispersed phylogenetic relationships among each other. And can not be significantly different from containing sets of genomic A Germplasm characteristics. Red bananas (AAA), suigeneris; Yunnan BB (BB) and the Dongguan high plantains (ABB) in the two closer relationship; Pisang Ceylan (AAB) and FHIA-18(AAAB) have the closest relationship, may be synonym.(4) Banana MSAP analysis28banana genomic DNA methylation rates in the diploid total methylation rate between15.8%-18.4%, the average rate of16.5%, triploid total methylation rate of16.7-19.7%between the average rate of18.3%, the tetraploid total methylation rate between15.8%-19.0%, the average rate of18.0%.28Banana methylation level analyses to compare the methylation rates and methylation rates did not show trends related to the ploidy. The hemimethylated rate is showing a triploid, tetraploid, is generally higher than the trend of the diploid.In summary, the reference to the banana drought physiological characteristics, combine banana genetic characteristics, can be seen:the number of chromosomes with the body can be used as a reference to one of the indicators that distinguish between A and B genome; the FCM individual germplasm and Simmonds identification of ploidythe morphological classification is inconsistent; clustering AAA type germplasm genetic differences, the B-genome phylogenetic relationships among each other, not significantly different from the sets of genomic A germplasm characteristics; and the level of DNA methylation showingtriploid and tetraploid than diploid trend. Of banana Drought Resistance and genetic characteristics related to that type of banana genome and its drought resistance is not a one to one relationship. Reasch show that the drought resistance of banana is not only related to their type of genome and its ploidy and so on.