Soybean Fusarium Root Rot Pathogen Species And Soybean Germplasm Screening

Fusarium root rot is one of the most devastating diseases of soybean throughout soybean-growing regions all over the world. This classical soil-borne disease is a dangerous, recalcitrant and notorious disease and has induced losses in yield and seed quality in main soybean production regions in China. Utilization of resistance varieties is the most economical and environmental safe method for controlling this disease. The keys of breeding for resistance are rational using of resistance, screening and searching new sources of resistance. Therefore, we should pay attention to the screening of the resistant resource to obtain high resistant varieties.In this study, the author collected soybean root rot disease samples in Jiangsu Province, Anhui Province, and purified the pathogen separations identified as Fusarium, and then determined their pathogenicity. Virulence strains were studied on their morphological and molecular biology characteristics to identify their specific taxonomic status. In this research, we separated317Fusarium strains, and28strains with virulence. Respectively,15strains of Fusarium proliferatum;5strains of Fusarium solani;6strains of Fusarium equiseti;2strains of Fusarium oxysporum. At the same time, we found that F. proliferatum may infect soybean causing soybean root rot, and cause serious harm. F. proliferatum as a new pathogen of soybean root rot has been rarely reported at home and abroad. The disease characteristics, pathogen identification and characteristics of F. proliferatum is important for disease prevention and control. In this experiment, we isolated F. proliferatum from soybean root, studied its morphological characteristics and pathogenicity, and through the ITS sequence determination and PRO1/2specific primers validation, further clarify its taxonomic status.152soybean germplasm candidate resources were evaluated for their responses to F. proliferatum strains TS306, the results showed that4cultivars (2.6%) were immune to TS306;16cultivars (10.5%) were high resistance to TS306;35cultivars(23.0%) were resistance TS306. The resistance resources (I+HR+R) accounted for only36.1%of total resources, however,susceptible resources (S+HS) accounted for63.9%of total resources, the soybean F. proliferatum root rot resistance resources are not abundant, breeding disease-resistant varieties is particularly necessary.20soybean resistance (I+HR) varieties were evaluated for their responses to F. proliferatum strains TS306, MC108. HA109and GY101, the results showed that10cultivars (50%) were multiple resistance to the4tested strains.