Morphological Observation, RbcL Gene Sequences And Germination Types Studies Of Four Species,(Halymeniaceae, Rhodophyta) From China

When we carried out the analysis on species resources of the coast of Lingshui, Hainan province and Luxun park, Qingdao city, Shandong province, by the method of combining the morphology observation and rbcL sequence analysis, we found G. intermeidia Wang et Zhang is a new species distributing in Lingshui, Hainan province and G. orientalis S.-M. Lin et H.-Y.Liang is a new record in the Chinese mainland distributing in Luxun park. Auxiliary cells and carpogonial ampullae of G. intermeidia are Grateloupia type and NJ phylogenetic tree and MP phylogenetic tree also showed that G. intermeidia form a small Grateloupia subclade. G. intermeidia has the following morphological features:Thallus are gelatinous to cartilaginous in texture; branches at the base part is slightly constricted and has proliferation on the thalli; the distance of branches is wide below and narrow above and the terminal branches have claw-like bifurcation; cortex composed of two to three layers of outer round to oval cells and two to three layers of round-shape inner cells; ampullae composed of two to three orders of unbranched filament; the cell divided and mixed the filaments around it and connected to each other through pit to form a peel of cystocarp after fertilization. Otherwise, The study of the mode of sporogensis were used in G. intermeidia. We found that it was ’direct discal type’and made of homeotypic gametophyte, tetrasporophyte and carposporophyte as same as the most Grateloupia species. G. orientalis has the following morphological features:Thallus are gelatinous to cartilaginous in texture; cortex composed of five to six layers of outer moniliform cells and one to two layers of stellate-shape inner cells: ampullae composed of only two orders of unbranched filament; carposporangia distribute in the cortex except for the bottom of thallus.The study of the mode of sporogensis were used in recently collected G. droyphora Howe, G. chiangii Kawaguchi et Wang, from Qingdao. We found that G. droyphora is ’mediate discal type’. The discoid produced protonema in a certain stage and the mature protonema can form to discoid. At last, the discoid continue to develop a complete thalli. G. chiangii was’direct discal type’. Development can be divided into three stages:mitotic, discoid, seedling. The development of the mode of sporogensis betwteen carpospores and tetraspore are identical.