Study On The Sanguinarine Against Bacterial Biofilm

As feed additives, antibiotics have effect on livestock health and growth. But they kill bacteria, while screening the role of drug-resistant strains. The abusing of antibiotics promotes the constant mutation of bacteria, so far as to form a biofilm (bacterial Biofilms BBF). The drug-resistant strains can in animal residues, thus affecting the security of animal food. In addition, the managements of the developed countries are increasingly stringent, Nordic even banned antibiotics. What’s more, they detected the drug residues of import and export food and used the drug residues as barriers greatly limiting the internationalization process of China’s livestock products. In china,46.1%of the antibiotics are used in animal husbandly and aquaculture. so there is an urgent need to develop new green substitutions in animal husbandly and aquaculture, which have no drug residues, no pollution, hard to produce drug resistance, and can effectively remove the biofilm.In China, Sanguinarine is rich in resources. It is readily biodegradable after use,has less environmental pollution and higher toxic to bateria. EU has already explored the Natural plant extract such as Sanguinarine instead of antibiotics. Thus the the research and development of sanguinarine as forage additives has important significance and broad market prospects.This paper analyzes the inhibitory effect of Sanguinarine in vitro, builds the bacterial biofilms of Salmonella paratyphi A,Staphy/ococcus aurus, Escherichia coli, focusing on the study of the influence on pathogenic biofilms of Sanguinarine in order to lay the foundation for Sanguinarine as a green feed additives. The main contents and results of this study are as follows:(1) Sanguinarine antibacterial test in vitro. Test the minimum inhibitory concentration of Sanguinarine on the pathogens (Staphylococcus aureus,invasive Escherichia coli, Salmonella paratyphi A, Bacillus cereus) and non-pathogenic bacteria(Bacillus bacillus, Bacillus coagulans) by tube double dilution method. Study the Sanguinarine’s antibacterial activity and compare with Chlortetiacycline which is allowed to add by oxford plate assay. It is found that the antibacterial activity increases with increasing concentration. Sanguinarine antibacterial circle diameter is the largest which is0.32g/L, and its inhibitory effect is better than chlortetracycline.(2) Build the bacterial biofilm.Use Congo red plate method, silver staining and crystal violet staining to study the fonnation of pathogenic bacteria biofilms of Salmonella paratyphi A, Staphylococcus aurus, Escherichia coli. The results show that the three kinds of pathogenic bacteria biofilm are successfully constrcted.(3) Reseach the influence of Sanguinarine to Salmonella paratyphi A, Staphylococcus aurus, Escherichia coli biofilm and the effect on exopolysaccharides. The results show that Sanguinarine has a greater impact on Salmonella paratyphi A biofilm and Escherichia coli biofilm than Chlortetracycline by Congo red plate;Through plant count for bacteria colonies, the number of Staphylococcus aureus, invasive Escherichia coli and Salmonella paratyphi A on biofilm are significantly decreased by0.16g/L sanguinarine; Sanguinrine can greatly reduce the exopolysaccharides on the biofilms; Through chemical identificating of the three kinds of biofilms, we find they contain alginate; Alginate is reduced after Sanguinrine by spectrophotometry determination. It is proved Sanguinarine has an impact on the role of bacteria biofilms and extracellular polysaccharide; At last, we can observe that Sanguinarine causes the variation of the biofilm structure with atomic force microscope and scanning electron microscope;...