Induced Defense Responses Of Wolfberry(Lycium Barbarum Linn) By Exogenous Jasmonic Acid And Aceria Pallida Keifer

The effects and mechanism of wolfberry after exogenous jasmonic acid (JA) induced and damaged by A pallida Keifer were studied. The changes of protease inhibitorsn(PIs), superoxide dismutase(SOD), phenylalanine ammonia-lyase(PAL), polysaccharide and xylogen of wolfberry before and after exogenous jasmonic acid(JA) treatment and A. pallida Keifer damage were tested. The results showed that:1. The activities of PIs in leaves of wolfberry increased significantly after exogenous JA treatment and damaged by A. pallida Keifer and it was influenced by JA concentration and time. The activities of CI inceased significantly after A. pallida Keifer damaged. The biggest increases were on the5th day and it was6.561U and12.728U before and after the treatment, respectively.2. The content of polysaccharide in leaves decreased significantly after exogenous JA treatment. The higher the concentrations of exogenous JA, the more it reduced. Polysaccharide of wolfberry leaves treated with0.1mmol/L JA decreased from1.233%to0.438%before and after treatment. The content of polysaccharide damaged by A. pallida Keifer was reduced and there was no increasing trend till the10th day. The max increased content of xylogen of wolfberry leaves was69.9%, which treated by0.1mmol/L JA.3. The activities of SOD and PAL increased significantly after the JA treatment and A. pallida Keifer damaged. The activities of SOD increased from70.77U to128.98U and PAL increased from10.91U to20.59U on the5th day. Both of them decreased on the10th day but still higher than control.4. The content of alkaloid and phenolic compounds in leaves of wolfberry increased significantly after exogenous JA induced and damaged by Aceria pallida Keifer.It is similar to that the content of betaine and atropine change trend of the wolfberry after exogenous JA induced and damaged by Aceria pallida Keifer. The content reached to the highest in treated1th day. The two alkaloids were both lower and reached to the lowest in treated10th days. The change tendency of choline chloride content of wolfberry first increased and then decreased.and then increased. It is different than other two alkaloids.The content of the8phenolic acids changed from different patterns after the treatment. In group CH,there was a similar trend of change in the content of caffeic acid, phenylacetic acid and syringic acid and reached to the highest in treated5th days; the changes of chlorogenic acid, ferulic acid, benzoic acid, salicylic acid and coumarin acid were similar and reached to the highest in treated1th day. These5phenolic compounds were decreased with the continuous treatment and reached to the lowest in treated10th days. In group L, it is similar to that the content of chlorogenic acid, caffeic acid, ferulic acid and coumarin acid change trend and reached the highest in treated1th day. These4phenolic compounds were decreased with the continuous treatment; the changes of phenylacetic acid, benzoic acid, salicylic acid and syringic acid were similar and reached the highest in treated5th days. In group H, the changing trend of chlorogenic acid, benzoic acid, phenylacetic acid, salicylic acid and syringic acid were almost the same and reached the highest in treated5th days. The trendeny first increased and then decreased; there was a similar trend of change in the content of caffeic acid, ferulic acid and coumarin acid and reached the highest in treated1th day. These3phenolic acids were decreased with the consistent processes.5. The wolfberry leaves can release a variety of volatiles after exogenous JA induced and damaged by A pallida Keifer.The results shows that there are obvious changes on components and content compared with the control.