| Higher plants at the top of the development and differentiation of lateral organs is veryimportant biological problems, also related to some crops and economic crops plant andeconomic coefficient of harvesting organs.And WOX gene family is a kind of characteristic ofthe transcription factor, which primarily at the top of the plant growth, namely stem tip meristemand root tip meristematic tissue expression regulation and control role, is very important for plantgrowth and development, become a research hotspot in recent years.This experiment was on the basis of WOX1gene clone in Chinese fir, WOX1genepromoter was cloned and analyzed. Through transgenic experiments, studied the functions andexpression patterns of WOX1with its promoter.Have achieved major results are as follows:1.By Tail-PCR,we cloned1.5kb WOX1gene promoter sequences, called pClWOX1.Onlinecomprehensive analysis showed that the promoter is a conservative cis element, but also containspecific of anther, pollen express components, stem tissue specific expression components,vascular tissue specific expression components, hinted that the function of its organization, organspecificity expression.2.Constructed plant expression vector pClWOX1:: GUS and pClWOX1:: YFP, viaAgrobacterium mediated method, tobacco transient expression and stress treatment found thatlow temperature, high salt, sucrose and glucose can inhibit expression of promoter activity,30%PEG can enhance promoter activity.Conducted a preliminary study on the conversion ofArabidopsis thaliana,screening for T1generation, positive Arabidopsis seedlings for GUShistochemical staining and fluorescence microscopic observation, found pClWOX1promotermainly expressed in the flowers; young leaves,fruit pod handles and fruit pods have a littleexpression;other parts were not detected expression.3.The WOX1excessive expression in Arabidopsis T1generation found that plant small,lateral node repeat similar rosette leaf type development mode, its collateral increase andcollateral fruit pods smaller.Preliminary analysis of the above problem, laid the foundation for revealing the fir WOX1gene expression regulation mechanism, and also has certain guiding significance for researchingof forest tree molecular breeding. |
