| With the rapid social-economic development,China’s papermaking industryhas also entered into a period of rapid growth.But in the paper making industry,removing lignin is an important source of environmental pollution.Therefore it isvery important to choose plant materials with low lignin content and/or lignineasy removal for the papermaking industry and environmental protection. In thisstudy, C4H,C3H and CCR genes were cloned from the young shoot ofNeosinocalamus affinis throught RACE and RT-PCR technology.And it was tomake bioinformatics analysis and tissue expression analysis for the threegenes.The main results of this study are as follows.NaC4H, NaC3H and NaCCR1were cloned from the young shoot ofNeosinocalamus affinis by RACE and RT-PCR technology. Their accessionnumber are JN571418,JF693629and JQ669677in GenBank, respectively.The results of bioinformatics analysis showed that NaC4H and NaC3Hbelong to the cytochrome CYP450family protein. Subcellular localizations ofthe two proteins were in the endoplasmic reticulum (membrane). The secondarystructures and tertiary structures were abundant in alpha helixs and randomcoils,beta turns and extended strains were less. The relative conserved disordereddomains were found in amino acid sequences encoded by NaC4H and NaC3Hgenes from Neosinocalamus affinis.The results of semi-quantitative RT-PCR analysis showed that NaC4H andNaC3H genes were differentially expressed in the different parts ofNeosinocalamus affinis.The expressions of NaC4H gene from the middle sectionand the bottom of shoots, the upper, middle section and bottom of stems werestronger than those of the unexpanded leaves, upper expanded leaves and uppershoots. The expressions of NaC3H gene from the unexpanded leaves,upperexpanded leaves, the middle section and bottom of shoots, and the middle sectionand bottom of stem were stronger than those of the upper shoots and upper stems. |
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