Regulation Effects Of Endophytic Bacteria On Aluminum-resistant Improvement Of Tea Tree

Fifty three aluminum-resistant endophytic bacteria were isolated from the healthy tissues of tea tree. Among those strains, G1,G3,G4was selected for further studding based on the evaluation of growth-promoting capabilities. This strain exhibited remarkable plant-growth-promoting properties including phosphate solubilization activity, production of phytohormone, nitrogen fixation, siderophore biosynthesis and1-aminocyclopropane-l-carboxylate deaminase activity. Moreover, the G3strain was also proved to facilitate seed germination and seedling growth of wheat. The16srDNA sequence analysis suggested that G1strain was Enterobacter sacchari, G3strain was Burkholderia cepacia, and G4strain was Burkholderia seminalis. The adsorption of Al3+exerpiment with condtion of different time、PH and temperature were contducted. In addition, the experiment results of aluminum ion adsorption showed that the three strains can absorb Al3+effectively.The3aluminum-resistant endophytic bacteria was inoculated to Tie-guanyin tea tree. After50days, the plants were harvested. Plant physiological and biochemical indexes of soluble sugar content, soluble protein content, SOD activity, POD activity, MDA content, phosphor content, and potassium content was determined. The results showed that when G1was inoculated to tea tree, soluble sugar content did not change. Soluble protein content did not change. The phosphor content in root, steam, and leaf decreased25.75%,42.11%,18.53%respectively. The potassium content in root, steam, and leaf increased5.99%,5.09%,0.15%respectively. The SOD activity increased10.44%. The POD activity decreased6.38%. The MDA contents decreased26.35%. The phosphor content in soil decreased5.06%. The potassium content in soil increased2.08%. When G3was inoculated to tea tree, soluble sugar content decreased11.17%. Soluble protein content increased5.06%. The phosphor content in root, steam, and leaf decreased12.76%,13.00%,21.20%respectively. The potassium content in root, steam, and leaf increased12.56%, decreased1.70%, and decreased4.75%respectively. The SOD activity increased10.44%. The POD activity decreased20.21%. The MDA contents decreased22.30%. The phosphor content in soil increased32.68%. The potassium content in soil decreased2.60%.When G4was inoculated to tea tree, soluble sugar content increased10.68%. Soluble protein content increased1.01%. The phosphor content in root, steam, and leaf decreased18.56%,15.17%,24.80%respectively. The potassium content in root, steam, and leaf increased2.84%,3.48%,0.45%respectively. The SOD activity decreased12.17%. The POD activity decreased6.38%. The MDA contents decreased26.35%. The phosphor content in soil increased31.91%. The potassium content in soil decreased0.69%. When different strains were inoculated to tea tree, different effect was exerted on tea tree’s growth.When G1was inoculated to tea tree, exchangeable aluminum content in soil decreased30.77%. Acid-soluble inorganic aluminum content in soil decreased2%. Humic acid aluminum content in soil increased1.42%. Exchangeable aluminum content in root of tea tree increased66.78%. Exchangeable aluminum content in steam of tea tree increased52.43%. Exchangeable aluminum content in leaf of tea tree decreased7.12%. Acid-soluble inorganic aluminum content in root of tea tree increased50%. Acid-soluble inorganic aluminum content in steam of tea tree increased4.96%. Acid-soluble inorganic aluminum content in leaf of tea tree increased51.13%.When G3was inoculated to tea tree, exchangeable aluminum content in soil increased23.08%. Acid-soluble inorganic aluminum content in soil decreased19.46%. Humic acid aluminum content in soil increased0.78%. Exchangeable aluminum content in root of tea tree increased57.44%. Exchangeable aluminum content in steam of tea tree increased29.67%. Exchangeable aluminum content in leaf of tea tree increased45.20%. Acid-soluble inorganic aluminum content in root of tea tree increased23.76%. Acid-soluble inorganic aluminum content in steam of tea tree increased21.17%. Acid-soluble inorganic aluminum content in leaf of tea tree increased35.75%.When G4was inoculated to tea tree, exchangeable aluminum content in soil decreased15.39%. Acid-soluble inorganic aluminum content in soil decreased11.11%. Humic acid aluminum content in soil increased24.57%. Exchangeable aluminum content in root of tea tree increased67.13%. Exchangeable aluminum content in steam of tea tree increased52.40%. Exchangeable aluminum content in leaf of tea tree decreased3.10%. Acid-soluble inorganic aluminum content in root of tea tree increased100%. Acid-soluble inorganic aluminum content in steam of tea tree increased26.13%. Acid-soluble inorganic aluminum content in leaf of tea tree increased33.03%. When G1, G3, G4were inoculated to tea tree, the total aluminum content in tea tree was increase remarkably. All the data above shows that G1, G3, and G4can promote the ability for tea tree to absorb aluminum.