Applications Of Three Dimensional Ordered Nanostructure For Biomolecular Migration And Separation

With the development of science and technology, biomolecular as the mainstream ofbiological products must be separated and purified from a mixture or analyzebio-molecules. Its using peculiarity and characteristic set a great demand on biomolecularseparation. So it becomes pretty important to develop efficient bioseparation technology inthe field of engineering technology. Compared with gel used in traditional bioseparationmethods, three dimensional ordered nanostructure as the separation system possessesordered structure, good connectivity, inexpensive, and color characteristics. In addition, itis easy to integrate in multi-step bioseparation technology through combining withmicrofludic technology and the resultant system may separate biomolecular efficiently andfast.In this work, we introduced three dimensional ordered nanostructure instead of gel inthe separation and purification compared with traditional bioseparation methods. Usingmicrofulidic technology, the colloidal crystal consisting of three dimensional orderednanostructure had been prepared by the self-assembly of colloidal sphere (SiO2\PS) incapillary and microchannel, respectively. Moreover, the silica inverse opal structure hadbeen fabricated after calcination in glass and capillary. Among these obtainednanostructures, colloidal crystal in microchannel had been used to study the biomolecularseparation and purification.Furthermore, COMSOL Multiphysics had been employed to simulate thebiomolecular separation and purification in the microchannel.. To establish crossmicrochannel and shape-array-microfissure, coupling electric field, flow field and surfacereaction\particle tracer, the migration and separation of DNA biomolecular had beenresearched in macro and micro field. The calculated results of concentration within twoelectric field in cross microchannel agreed well with the experimental results. Inshape-array-microfissure, the more periodic structure was, the more complex velocity distribution was observed in the more periodic structure. In complete periodicshape-array-microfissure, using time setting to control the amount of biomolecular throughseparation channel, the migration and separation of DNA biomolecular had been simulated.In the future, optimizing the parameters to get close to those in experiment, such as charge,mobility and radius, the simulation will be a good guide to the bioseparation experiment.