Separation, Characterization And Properties Of Collagen From Changbai Mountain Rana Chensinensis

This paper is Supported by Project of National Key Technology Research andDevelopment Program for The12th Five-year Plan（NO.2012BAD33B03）.With the RanaChensinensis as the raw material preparation of functional food in our country, such asrana chensinensis oil, rana chensinensis spawn, these products favored by consumers.1. The results contained: proteins contents of38.2%, fats contents of4.8%, ashescontents of8.1%and water contents of48.5%. Enzymolysis conditions were optimized toprovide foundation for authentication and properties research. The experiments resultswere pH1.966, enzyme concentration of5.42%, liquid-solid ratio of145.2:1, under theoptimized condition, the collagen extraction yield was29.65%.2. The results indicated the samples were collagen confirmed by High PerformanceLiquid Chromatography (HPLC). Differential Scanning Calorimetry (DSC) was used tocontest the degeneration temperature38.62℃~46.48℃. Field Emission Scanning ElectronMicroscopy (FESEM) was applied to observe the morphology of samples.3. The experiments results showed the water-retaining properties could reach75.8%.The moisture rate of depuration collagen could be43.2%under R.H43%. The moisturerate of depuration collagen could be67.4%under R.H81%.4. In the process of antioxidant experiments, the results discovered metal ions have stimulativeeffects on collagen antioxidation. Therefore, on this basis, collagen experiments were chelating withmetal ions. Combining single experiments with the ternary quadratic regression orthogonal designexperiments, the optimized condition was pH3.185, salt rate of4.025:1, time of31min, withzinc sulfate. Under the optimized condition, the chelating rate could reach95.7%.