Application And Analysis Of The Interaction Between The Folic Acid, VitaminK3, Heparin And Melamine, Rhodamine6G

This article uses the fluorescence spectroscopy studied, such as drug molecules（Folic acid,VitaminK3,Heparin） with fluorescent substances (Melamine,Rhodamine6G)spectroscopy characteristics of the interaction. Drug molecules is studied in thefluorescence quenching mechanism of some of the fluorescent material. Describes theexperimental condition, the drug molecules of melamine and the influence of thefluorescence spectra of rhodamine6G, established the optimum experiment conditions,determine the content of drug target of drugs, established measuring drug concentration ofnew fluorescent method, applied to the determination of drug content in the actual samples.The paper mainly includes the following works:1By use Melamine as a fluorescent (PL) probe，folic acid was quantitatively detectedbased on PL quenching Melamine.The influences of concentration of Melamine and theexcitation wavelength，incubation time were investigated and the results indacated that inB-R8.36and10min of incubation，the folic acid concentration within a range of0.1-12mg/L had a good linear relation. The obtained linear fitting equationis:F0/F=4.9696+10.48862c （mg/L)with the correlation coefficient of0.9988. Thedetection limit is0.044mg/L.2In pH7.04buffer medium, Vitamin K3can react with melamine to form complexes,which lead to fluorescence quenching of melamine, and the wavelengths of fluorescenceexcitation and emission of melamine were256nm and366nm, respectively. Fluorescencequenching value was proportional to the concentration of Vitamin K3in a certain range.A novel fluorescence quenching method for the determination of Vitamin K3wasestablished. The linear range for determination of Vitamin K3is0.1-10mg/L. Thedetection limit is0.085mg/L. The method has been applied in the determination ofVitamin K3in real sample with satisfied results.3In pH8.0buffer medium, Heparin can react with Rhodamine6G to form complexes,which lead to fluorescence quenching of Rhodamine6G and the wavelengths offluorescence excitation and emission of Rhodamine6G were482nm and560nm,respectively. Fluorescence quenching value was proportional to the concentration of Heparin in a certain range. A novel fluorescence quenching method for the determinationof Heparin was established. The linear range for determination of Heparin is0.05-3mg/L.The detection limit is0.0061mg/L. For six parallel determinations, the relative standarddeviations were1.1%~1.9%and the recoveries were98.76%~102.6%. Application offluorescence spectrum method to study the interaction between Heparin and Rhodamine6G and quenching mechanism has carried on the preliminary discussion. The method hasbeen applied in the determination of Heparin in real sample with satisfied results.