Effect Of Milk Powder Rich Of Opioid Peptides On Human Intracellular Communication Networks And Its Evaluation On Anti-fatigue Action

Opioid peptides, a kind of anti-fatigue peptides, have a deeply effect on improvedhuman body function, relieve fatigue, ameliorate sleep situation and so on. This topic ismainly about the powder milk rich in opioid peptides. The preparations of opioid peptidesare produced by hydrolyzed casein using pepsin. Then put the debitterized hydrolysatesinto powder milk, formulated the powder milk with the effect of anti-fatigue. This researchis based on the previous study, all the former study have been determined the powder milkwith opioid peptides has the function of anti-fatigue by animal experiments. This study ismainly about: pilot test of powder milk which is rich in opioid peptides; edible test byrecruiting volunteers in order to determine the human cytokines and the questionnaire asaddition. All by above, evaluated the anti-fatigue effect in powder milk more accurate,comprehensive, and humanity.The pilot test of powder milk with opioid peptides produced about2kg enzymatichydrolysates from casein, and25L enzymolysis liquid each batch；a total of10batches.According to the optimized conditions of enzymatic hydrolysates from casein tohydrolyzed protein, conditions as following: the substrate concentration5%, enzyme isadded as25,000U/3g substrate, pH=2.5,43℃as the enzymolysis temperature. After thehydrolysis, using β-cyclodextrin (β-CD) debitterized, the condition is: the concentration ofenzymatic hydrolysates from casein is0.6%, concentration of β-CD is2.5%,40℃as thetemperature of debitterizing,30min stirring reaction. Then desalination and concentrationproduced the powder of enzymatic hydrolysates from casein by freeze drying. According tothe formulate: each100g powder of milk add4.2g enzymatic hydrolysates from casein,17gβ-CD, and mass fraction of1%sucrose to premixed opioid peptide powder milk.Research the effects to human body cellular network communication by full of opioidpeptides. Recruiting young healthy volunteers as the experiment object divided thevolunteers into3groups with: add enzymatic hydrolysates from casein group(Casomorphin); no add whole milk powder; and add opioid peptides as formula milk, eachgroup has10volunteers. Experimental need2days as total, the first day as a control case,all the volunteers take the same food, work and rest regulated, any outside food is forbidden,do not take any major physical sports.3hours after the lunch take4mL blood samples ascontrast ones. All the contrast samples should be let stand4hours in4℃, and under thesame tempters situation to centrifugal separation and repackaging, keep in-70℃for thefollowing needs. The following day, all the volunteer take the same regular of the first dayin working and rest, after the lunch, as the dividing condition instruction, take each kinds ofpowder milk separately: enzymatic hydrolysates from casein (Casomorphin)4.5g/person; whole milk powder25g/person; and powder milk rich in opioid peptides25g/person. After3hours take4mL blood samples, with the first day processing preserved under test. UsingMillipore Kit and liquid chip scanner to detect the before and after group’s concentration of38kinds of cytokines in serum, using paired sample T test method to deal with testing data.According to the result,7kinds of cytokines in cytokines have significant changes,respectively, IP-10, VEGF、IL-17A、IFN-γ、G-CSF、IL-1ra and TNFα. Get the7kindscytokines information by online encyclopedia (http://www.copewithcytokines. de/cope.cgi),according to the synthesis and secretion of cells and target cells data, constructed and bodyafter eating the subject’s content variation of cell communication networks.Observed from a cellular communications network diagram, all cellularcommunication networks can be roughly divided into three layers, natural killer cells,Sertoli cells, hepatocytes located in the first layer, the cells through the way such asendocrine system, nervous system, and so on. Once excite by signal, command other cellsto make corresponding response. T-cells, monocytes, B-cells, T-helper cells and thymuscells, neutrophils, macrophages, fibroblasts and epithelial cells, glial cells, smooth musclecells, endothelial cells, keratinocytes, synovial membrane cells, stromal cells and follicularcells and corpus luteum cells are located on the second floor of the hierarchical network,bear the mission of signal, among them as a necessary part, pass signals to more target cells.Langerhans cells, osteoclasts, hematopoietic progenitor cells, fat cells and islet cells,progenitors of leukocytes and lymphocytes as target cells in the networks, received thesignal transmission from the first two levels of command, to make corresponding responses,as effector cells play a biological function. Through changes in cellular communicationsnetwork, can visually analyzing communication between cells before and after eating thefood law, speculated that the mechanism is: rich in opioid peptide after milk powder intothe intestinal immune system through cellular network adjustment, can alleviate balancewas destroyed due to the immune system and cause physical discomfort, such as caused byfatigue of colds, inflammation of the digestive system, etc. Most notably, fat cells and glialcells are involved in signal transduction process, and further illustrate opioid peptidepowder is rich in the mechanism of action of fatigue and promote sleep and way.For more detail, take the high school students of Beichen in Zhuolu county,Zhangjiakou, Hebei province as the sample. In order to acquire the anti-fatigue effect withopioid peptides powder of the volunteers, using questionnaire for a tracking back in30days.All the volunteers take20g powder milk rich in opioid peptides2to3hours before they goto sleep, and take a questionnaire each day as recording. After the experiment, the screeningof the questionnaire was carried out on the data. Using paired samples T test and nointeraction of two-factor variance to analysis. Results showed that: the powder milk rich in opioid peptides has a significant effect on relieve fatigue and sleep improvement.Above all, the study results powerful certified that: powder milk rich in opioidpeptides have a deeply effect on anti-fatigue and improving sleep.