Application Of Matrix Solid Phase Dispersion Extraction Capillary Zone Electrophoresis In Determination Of Flavonoids And Ginsenoside

Ginsenosides are the main active constituents of ginseng, and play an importantrole in promoting human health. So ginsenosides have attracted more and moreattention in recent years. Ginsenosides can regulate the central nervous system,improve the immune system and the functional capacity of the heart, enhance thebody’s excitability and adaptability. With the development and sale of a variety ofginseng products, the study of the ginsenosides is necessary. Flavonoids are a groupof secondary metabolites that present in plants. The flavonoids are beneficial tohealth and have various pharmacological activities, such as antioxidant, anticancer,antimicrobial and so on. In this thesis, the matrix solid-phase dispersion (MSPD)extraction capillary zone electrophoresis was applied to determination of theginsenosides and flavonoids.The MSPD was used to extract the ginsenosides, and the conditions of theMSPD were investigated and optimized. Diatomite was used as the dispersant and12.00mL of50%aqueous methanol was used as the elution solvent. The absorbanceof the ginsenosides Rg, Rb1and Re was measured at200nm. The results showedthat when the background electrolyte (BGE) was30mmol·L-1borax and10%methanol was used as organic additive，the ginsenosides can be effectively separatedfrom each other. The analytical performances of the present method were studiedand the real samples were analyzed. The recoveries for the three ginsenosides werebetween89.03105.02%.MSPD was applied for the extraction of flavonoids. Florisil was used as thedispersant and methanol was used as the elution solvent. The flavonoids includingkaempferol, rutin, quercetin, luteolin, ferulic acid and naringenin were extractedfrom the flower of Chrysanthemum morifolium Ramat.. When the elution solventvolume and flow rate were8.00mL and0.50mL·min-1, respectively, the extractionfields were maximum. Boronic acid-potassium dihydrogenphosphate solution wasused as the BGE. When the pH of the BGE was10.0, eight kinds of flavoniods were separated from each other completely. In the experiment, the standard curves wereconstructed. The limits of detection for determining the analytes were in the rangeof46.4972.03ng·mL-1. The relative standard deviations varied between1.41%and3.40%(n=5). The real samples were analyzed. The recoveries for determining theflavonoids were in the range of80.13121.25%.