Studies On Compound Glucosamine And Chondroitin Tablet

In this paper,the development of drugs for curing osteoarthritiswere reviewed.Through single-factor experiment and orthogonalexperiment, According to the composition of raw materials and the ratioby determining by efficacy experiment, through the single factorexperiment and orthogonal experiment to determine the prescription andpreparation process of compound glucosamine and chondroitin sulfatetablet, As a index to the tablet appearance, granule flowability anddisintegrated time of tablets.In this paper,the quality standard and preliminary stability ofcompound glucosamine and chondroitin sulfate tablet were studied. Thecontents of glucosamine hydrochloride and chondroitin sulfate in tablethave been determined synchronously by HPLC. The results showed thatusing acetonitrile-10mmol·l-1sodium pentanesulfonate solution(10:90) asthe mobile phase,the flow rate was0.8ml·min-1,column temperature was25℃and detection wavelength was192nm, the number of theoreticalplates were respectively calculated according to chondroitin sulfate A andglucosamine hydrochloride peak are not less than3000,and the degree ofseparation is good. The linearity was in the range of25μg·ml-1～300μg·ml-1for chondroitin sulfate,and125μg·ml-1～1500μg·ml-1forglucosamine hydrochloride.The RSD of precision and stability forglucosamine hydrochloride and chondroitin sulfate were all less than2.0%. The average recoveries (n=9) for them were100.1%(RSD=0.96%)and99.8%(RSD=0.74%), respectively. The results showed thatthis method can be used for determination of compound glucosamine and chondroitin sulfate tablet. According to the dissolution curve, it isdetermined that the time of dissolution was30minutes and the limit ofboth was80%oflabelled amount.The preliminary study of stability were done. Accelerated test resultsof6months and long-term test results of9months showed that all of theexamined items were eligible, which showed the stability of the tablets isbette.The result of this paper shows that preparation process of the tabletis reasonable and feasible, quality is stable and controllable.That providethe basis for the development of compound glucosamine and chondroitinsulfate tablet.