Extraction Of Total Flavonoids From Horsetail And Bioactivity Research

This study belongs to the part of jilin province science and technologydevelopment plan "functional egg industrialization production demonstration" project(20100243), The horsetail grass was raw materials with experiment, using theultrasonic extraction, supercritical CO2extraction technology to optimize theextraction technology of total flavonoids in scouring rush parameters, thus horsetailflavonoids of oxidation experiment and cholesterol in vitro experiment, the results areas follows:(1) In medium yellow ketone compound extract, horsetail grass through orthogonalexperiment, the ethanol concentration, extraction time and solid-liquid ratio andextraction temperature on the impact of four factors on flavonoids extraction quantityorder as: primary and secondary extraction temperature> solid-liquid ratio>extraction time> ethanol concentration, and the optimal conditions for the extractionof D2C1A2, B2: extraction temperature40℃, liquid ratio of1:20, extracting timefor40min, ethanol concentration is70%; Using ethanol extract of sample dissolution,with ethanol as the reference, in the ultraviolet visible light wavelength of220nm to220nm, ultraviolet spectrum scanning to get samples of UV spectra, preliminarilydetermine the type of horsetail flavonoids flavonoids and flavonols.(2) The extraction of horsetail flavonoids was studied by supercritical fluid CO2,regression equation is obtained as follows:Y=13.76+2X1-2.81X2+0.06X3-0.05X1*X2-0.35X1*X3+0.78X2*X3-3.84X12+1.33X22-3.07X32, the extraction pressure is found:34.8MPa,extraction temperature is36.6℃, with the volume of36.6mL, achieve maximum13.98%. Although flavonoids table content extraction rate is low, but extract pure, golden color, it is fragrant, fat, and less solvent residual, effective components fromdamage and oxidation.(3) The horsetail antioxidant with coarse extraction of flavonoids was researched, theresults show that when the mass concentration of total flavonoids horsetail140ug/ml,DPPH clearance can be up to61.62%of total flavonoids showed horsetail has morestrong scavenging effect on DPPH free radicals, horsetail flavonoids crude extract ofDPPH, HO, O2-clear ability and the ability of removal of sodium nitrite wereconcentration-response relationship, and were higher than the same concentration ofVCand citric acid, especially clear HO ability than VCand citric acid, the resultsshow that horsetail flavonoids crude extract has stronger antioxidant ability, and theantioxidant capacity higher than that of VCand citric acid.(4) The cholesterol micellar solution was researched by finally simulated, combiningthe cholesterol-lowering activity in vitro model, the experimental results show that thehorsetail alcohol extract of simulated cholesterol micelle solubility has certain effect,and combined with the effect is more noticeable, and with the increase of horsetailalcohol extract concentration, the combined with enhanced role, in10mg/mL~40mg/mL range present certain concentration-response relationship, when the concentration of horsetail alcohol extract40mg/mL,the combined rate of56.85%, because of the alcohol extract of the main component offlavonoids, so you can infer the horsetail flavonoids extract has certaincholesterol-lowering activity in vitro, but the activity of specific mechanisms needfurther test and research. This paper studies scouring rush further research is toprovide a certain basis, and to improve the utilization value of horsetail plant also hasa certain significance.