Study On Matrix In Different Samples By Desorption Corona Beam Mass Spectrometry

With the rapid development of ambient ionization, real-time detection and high throughput screening can be processing based on such technology. The advantage of ambient Mass Spectrometry is its detection does not need any sample preprocessing. However, no sample preprocessing will lead a significant influence on target compound because of complex matrix, which means that detecting such sample with complex matrix will possibly obtain false positive or negative results. In addition, different matrix in various samples has different influence on the detection via ambient Mass Spectrometry. Although desorption corona beam ionization mass spectrometry (DCBI-MS) can be applied into various areas, it is not widely used because of its non-commercial limited. In order to extend the DCBI-MS into other areas, it is much important to analysis the detection of different matrix samples with DCBI-MS. Therefore, in this paper, we analysis the detection on animal tissue matrix (animal-origin food), mixed matrix of plant extraction (Chinese patent drugs), and separation media matrix (silica-coated TLC plate). Specifically, based on DCBI-MS developed by Shimadzu Corporation, we take a fast detection on the antibiotics, sugar-reducing medicines and quaternary ammonium alkaloids in animal-origin food, Chinese patent drugs and commonly herbal. Meanwhile, we analysis the influences of three above matrix on the detection, and compare them to the results of non-matrix compounds.The main research contents are as follows:1. Animal tissue matrix analysis with DCBI-MS. We developed a novel strategy, and applied it into animal-origin food which mainly consists of pork and fish. The main task is to fast selection and even semi-quantitative detection on8antibiotics in these animal-origin foods. Specifically, in positive ion mode, results based on molecular ion peak area of quantitative show us that the semi-quantitative detection towards8antibiotics is reasonable. The method showed good linear coefficients in the range of0.5000-125.0mg·L-1for all analytes. The limit of detection was lower than0.084μg/mL. Furthermore, through comparing the MS spectrum of standard mixed solution to the matrix matching solution, we can find that suppression of ionization efficiency has no influence on detection of8antibiotics. Because of simple preprocessing and fast detection of DCBI-MS, as a result, it can be applied into real-time detection.2. Analysis of mixed extraction matrix in plant by DCBI-MS. To develop a rapid method for detection of sugar-reducing medicines (nateglinide, dimethyldiguanide, voglibose, glipizide, pioglitazone, repaglinide) with the desorption corona beam ionization mass spectrometry (DCBI-MS) technique. The DCBI condition including temperature was optimized according to the resulting mass spectra intensity by positive ion mode. The method showed a good linearity in a given range for all of analytes. The linear coefficients of analytes were higher than0.98. The LODs of6sugar-reducing medicines were lower than0.03851mg/L. The developed DCBI-MS is accurate, sensitive for fast screening and semi-quantification of the sugar-reducing medicines in Chinese patent medicines and it provides the basis for further accurate regulation test.3. Separation media matrix analysis with DCBI-MS. A novel rapid method by coupling derivatization reagent with desorption corona beam ionization mass spectrometry (reactive DCBI-MS) for determination of10alkaloids (8quaternary ammonium alkaloids) in herbal medicines. In this method, we select the best reactive solution (saturated NaOH solution: methanol=3:7) for easily detecting quaternary ammonium alkaloids. The DCBI condition including temperature (220℃) was optimized according to the response of mass spectra peak area. All of the target molecules were detected within1min. Finally, we successful apply the reported method to6herbal via in situ analysis8quaternary ammonium alkaloids or combined TLC-DCBI-MS to detect4alkaloids in Mecleagua cordata (Willd) R.Br.. The method showed a good linearity of5-500mg·L-1for4alkaloids. The linear coefficients of analytes were higher than0.99. The developed derivatization DCBI-MS is simple, rapid and sensitive for qualification of the quaternary ammonium alkaloids in herbal medicines.