Preparation And Characterization Of Biomedical Films Based On TiO₂Nanostructures

TiO2nano films have been widely applied in biomedical filed as biomedical films due to their excellent biocompatibility and bioactivity. This work aims to improve the biological properties of TiO2nano films based on materials/cell interfacial reactions at a molecular level. In this work, TiO2nano films were prepared through hydrothermal or self-assembly method. Then CaP or RGD was grown on the films. SEM, XRD, EDS measurements were used to study the material properties of the films. Bovine serum albumin protein adsorption and in vitro cell culture experiments were used to evaluate the biological properties of the films. The main work and results are summarized as followings:1. Preparation and characterization of CaP/TiO2nanorod composite films and their osseointegration propertyTiO2nanorod films were prepared by a hydrothermal method. Then CaP was deposited electrochemically. Porous CaP/TiO2nanorods composite films were obtained. Bovine serum albumin protein adsorption characterization showed that the films have improved protein adsorption properties. In vitro cell culture experiments also showed CaP/TiO2nanorod composite films have good bioactivity and osseointegration properties.2. Preparation and characterization of RGD immobilized TiO2nanodot films and cell detaching performanceTiO2nanodot films were prepared by a self-assembly method. Then RGD peptides were immobilized on the films by a physical adsorption method. In vitro cell culture experiment demonstrated that RGD could improve cell adhesion and proliferation on the RGD immobilized TiO2nanodot films. Besides, cells on the films had a high level expression of vinculin proteins. Cell detachment experiment showed that cells could be detached from RGD immobilized TiO2nanodot films after UV365illumination with good cell viability. It was found that RGD could promote cell detaching from the films, that could be ascribed to RGD release on RGD immobilized films surface with UV illumination. After cell cultured for5days, cell sheets were formed on the films and could be detached after UV365illumination. Fluorescence staining results showed that cadherin proteins had a high level expression which means that cells in cell sheet have strong interactions with each other, indicating a promising application prospect.