| Background and purpose:Recent years, a growing number of studies have found that polyunsaturated fatty acids play a significant role in inhibition and relieve of the occurrence and development of all kinds of chronic diseases. There is latent risk of complication in chronic disease. At this stage, however, the study of PUFAs is to stay on the influence on single chronic disease; there are few of research about the effect on chronic complications.In addition, PUFAs are confined to treat the subjects alone, and did not involve combinated therapy. The excellent biological activity of PUFAs was not utilized to combine with traditional chemical drugs which have toxic side effects to explore the new way of low-toxic treatment. In this paper, related preliminary research and discussion has been carried on:1) In this paper, gastric cancer cells under the environment of high sugar and high insulin were treated with the typical PUFAs ALA (a-Linolenic Acid), LA (linoleic Acid). With the key point of oxidative stress, the effect of PUFAs on gastric cancer cell was studied.2) Gastric cancer cells were treated by the mixture of PUFAs and the traditional antitumor drugs5-FU, oxidative stress, migration and metabolic were investigated. This study aims to do a basic research work on PUFAs about the transition from research stage to application stage.Methods:1) Human gastric normal cell GES-1and tumorous cells, MGC, SGC were employed in the study. The model of high-glucose and high-insulin was established and treated with PUFAs (ALA, LA) for observing the effects of PUFAs on the growth of gastric cells. Cell proliferation was measured by MTT assay; the effects of ALA, LA on cell apoptosis was analyzed by flow cytometry; fluorescence intensity indicated its accumulation of ROS and kits were used to detect the level of SOD, MDA respectively. ELISA is the method for LXA4level;2) Human gastric cancer cell MGC, SGC were employed in the study.5-FU only as experiment control group, after48h intervention by6kinds of PUFAs, cell proliferation was measured by MTT assay, kits were used to detect the level of SOD, MDA and NO respectively; fluorescence intensity indicated its mitochondrial membrane potential;3) Human gastric cancer cell MGC, SGC were employed in the study.5-FU only as experiment control group, after48h intervention by6kinds of PUFAs, wound healing assay was used to measure migration, ELISA kits were used to determinate the level of Tumor Necrosis Factor a,(TNF-a) and vascular endothelial growth Factor (VEGF) and the expression of metabolism related Factor Peroxisome proliferator-activated receptor y, PPAR y, CCAAT-enhancer binding protein a (CEBPA) and Lipoprotein lipase (LPL).Results:1) ALA, LA made the activity of SOD decreased significantly, the accumulation of MDA, ROS increased significantly, two strains of cancer cells were significantly apoptosis, the level of LXA4dropped. ALA and LA could significantly increase the accumulation of free radicals, reduce the ability of scavenging free radicals, cause the oxidative damage of the cancer cells, and eventually lead to apoptosis. ALA and LA inhibited the growth of gastric cancer cell in high-glucose and high-insuli stress, and protected normal cells from damage at the same time.2)6kinds of polyunsaturated fatty acids, ALA, EPA, DHA, LA, GLA and AA were added into the culture to decrease the activity of SOD of gastric cancer cells; and the secretion of NO and the mitochondnal membrane potential significantly reduced as well; the accumulation of MDA was significantly increased, normal cells free from damage.3) The addition of6kinds of PUFAs could obviously inhibit migration of gastric cancer cell, inhibit the secretion of angiogenesis correlated factors TNF-a and VEGF, increased the expression of metabolism-related factor PPAR y, CEBPA and LPL. Polyunsaturated fatty acids combined with5-FU can effectively enhance the effect of inhibitor, and has certain protective effect on normal cells. |
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