Study Of Flavonols Molecularly Imprinted Polymer Film By Electrochemical Sensor

Natural flavonol, is the treatment of cardiovascular and cerebrovascular diseases,endocrine system disorders, and cancer drugs such as the main active ingredient, widelypresent in the plant roots, stems and leaves. A wide range of natural flavonoids, a variety ofingredients were born in the same plant, and high structural similarity, traditional purification,separation method is complicated time-consuming process, and the enrichment and separationineffective, content detection, quality evaluation difficulty. Therefore, the development ofefficient extraction, separation, it is very necessary to new method for detecting suchcompounds. This thesis molecular imprinting technique flavonol (quercetin, luteolin, morin,puerarin) imprinted molecule, o-phenylenediamine as a functional monomer, the use ofelectro-polymerization method to prepare a selection the good high detection sensitivityflavonols molecular imprinted poly-o-phenylenediamine film electrochemical sensors.This paper mainly carried out the following aspects:1. O-phenylenediamine as a functional monomer, quercetin as imprinted molecule, cyclicvoltammetry in the graphite electrode surface preparation-containing the imprintedmolecule of poly-o-phenylenediamine film by electrochemical methods imprintedmolecule elutionthe quercetin molecularly imprinted electrochemical sensors. Detailedstudy of the optimum experimental conditions of the preparation of the sensor molecularlyimprinted sensor imprinting efficiency, the number of binding sites and binding constantsand sensor specific selectivity of the imprinted molecule.2. Detailed study of the molecularly imprinted electrochemical sensors determination ofquercetin in experimental conditions, to establish a new method of differential pulsevoltammetry determination of quercetin, and the new method is applied to two Ginkgobiloba drugs quercetin determination recoveries between99.2%to102%.3. Using molecular imprinting technique o-phenylenediamine as a functional monomer forimprinting molecule, luteolin, morin and puerarin prepared by molecular imprintingsensor. Molecularly Imprinted blot efficiency of the sensor, the binding constant and the number of binding sites, and specific selectivity of the imprinted molecule, results showthat three molecular imprinting sensor has a good imprinting effect, and the choice of ahigher visibility.