Catalytic Synthesis Of Adoptor Molecule Oligopeptide

This study addresses the catalytic synthesis of man-made adoptor molecule, inwhich farnesyl transferase is used to catalyze the production of man-made oligopeptidecontaining farnesyl group. The man-made adoptor molecule, an important component inthe yeast-based cytometric array, is designed to mediate the conjugation of yeast cellswith antibodies. This study includes oligopeptide synthesis, reactive systemoptimization and adoptor molecular purification.The synthesized oligopeptide requires: the farnesyltransferase can catalyze theoligopeptide; the remaining acids have at least one carboxylic group; detective signal issensitive.The optimization of this reactive system included two steps. First, a single factorexperiment was employed to define the farnesytransferase appropriate temperature andpH. Second, an orthogonal test method was utilized to indagate the influences of threefactors comprising FPP concentration, farnesyl transferase concentration and thereactive time on the yielding rate of man-made adoptor molecule.Using gel column chromatography, farnesyl transferase was removed from thereactive system. The man-made adoptor molecules was obtained by vacuum-freezedrying and was stored at-80℃.