Characteristics On P Accumulation And Part Of Physiological And Biochemical Mechanism Of Pilea Sinofasciata

Water-soluble P from the runoff and leakage in soil is considered as the main source of aquatic environment eutrophication. which is one of the most important environmental problems worldwide. Using P accumulators to remove superfluous elements from soil or water-body serves as an attractive strategy. Experiments were conducted to investigate the characteristics on P accumulation and part of physiological mechanism of the mining ecotypes of Pilea sinofasciata in this study, compared with non-mining ecotype (NME). The main results are as follow:(1) P concentration and accumulation of the ME were firstly increased and then declined with ascending P concentration in the soils. The highest P accumulation of P. sinofasciata was observed when plant grown with400mg P (kg soil)-1.There was no significant difference between the shoot biomass of the ME and NME. Higher P concentration and accumulation were observed in the ME than NME for all treatments. Acid phosphatase and phytase activities in roots were elevated with increasing P supply levels, and the ME presented higher activities compared with the NME.(2) As growth progresses, significant increases in both shoot and root biomass were evaluated in ME. A similar increase in P accumulation was also observed in the ME. The highest P accumulation was141.49mg plant"’in shoot and34.71mg plant-1in root. After5,9and13weeks growth, shoot P accumulation was significantly higher than that of the NME. After13weeks growth. P extraction rate of the ME was1.13%in plant and0.91%in shoot.(3) The biomasses of the ME of P. sinofasciata were significantly higher than the NME. Biomasses of both ecotypes were firstly raised and then decreased with the P treatment increased. The highest biomass appeared on P400of both ecotypes.2) P concentrations of root, stem and leaf were increased with the P treatment raised of both ecotypes. P concentrations of the ME were higher than the NME. The highest P concentration of the ME was as high as22.85g kg-1.3) Total root length, total root surface area, total root volume and lateral root number of the ME were higher than the NME.With the P treatment raised, total root length, total root surface area, total root volume and lateral root number of the ME were firstly raised and then decreased. The average root diam of the ME and NME were not significantly different within high P supply, and the difference between ecotypes were not significant. It indicated that, the ME showed a better P accumulacation than the NME and a nice root morphological characteristic such as total root length, total root surface area, total root volume and lateral root number are important features to the high P uptake ability.(4) Biomass, P concentration and accumulation of the ME were increased with ascending organic P (pig manure) concentration in the soils. The highest Biomass, P concentration and accumulation of P. sinofasciata was18.2mg plant-1,5.09g kg-1and93.06mg plant-1, when plant grown with970mg organic P (kg soil)-1. P extraction rate and P removed from soil were increased with ascending organic P concentration in the soils, but enrichment coefficient decresed. The ME showed a significant advanced in shoot P concentration at970mg organic P (kg soil)-1and shoot P accumulation at both485and970mg organic P (kg soil)-1. P. sinofasciata planted can obviously decresed the water soluble P and available P. water soluble P were only38%and56%after planted at85and970mg organic P (kg soil)-1, while available P were only65%and80%.