| Monascus M739was taken as research object in this paper, the impacts of different carbon source and nitrogen source of Monascus on the production of color capacity and growth were studied by using liquid fermentation technology and the results are as follows:(1)When soybean peptone was used as nitrogen source medium, the rice flour, corn flour, sweet potato flour, dioscoreae flour, glucose, sucrose, or glycerol was added seperately as carbon source to study the color production capacity and cell growth of Monascus. The results showed that sweet potato flour was conducive to color production and the corn flour was the most conducive to biomass growth.The highest color value and dry weight of mycelium were19.93u/mL and1.98g respectively at the end of fermentation. When the sweet potato powder was used as carbon sources medium, the influence of peptone, yeast extract, NH4NO3and urea on the color production capability and the cell growth of Monascus was investigated. The result shows that soybean peptone is benificial to the color production and biomass growth and the color value and mycelium dry weight were23.8u/mL and1.76g respectively at the end of fermentation. In addition, Citrinin was not detected in fermentation broth of Monascus M739and this result indicated the fermentation product is safe.Analysis of the production of pigment and citrinin of Monascus M1under red light conditions showed that the red light can significantly stimulate biomass grow th and pigment production from2987U/g up to2376U/g. In addition, Aspergill us mycotoxins-citrinin production was monitored and discovered that citrinin produc tion was88μg/g in red light and150μg/g in dark condition. Production of Mona scus was6.26μg/mL in red light and4.96μg/mL in dark condition.Therefore,re d light is able to significantly reduce citrinin production improve the Monacolin K production, which may provide a new way to reduce citrinin production and impr ove the Monacolin K production of Monascus. |
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