| In the introduction, the background and significance of reach was introduced. Definitions, qualitative and quantitative methods of gas chromatography was also introduced and the main fields of application of gas chromatography was reviewed. Moreover, the principles and applications of ICP-AES and applications and characteristics of Hydride generation method was briefly introduced. The research status and progress of determination method for ethanol and trace elements in the blood were elaborated.The determination method of blood ethanol content by headspace gas chromatography was established and the influence of blood preservation conditions on the ethanol content was briefly discussed. Adopted manual headspace to inject sample, discussed and selected the best equilibration time and temperature, and did condition experiments for the column temperature of gas Chromatograph. This was the best solution:precisely drow the blood1.0mL, then added tert-butanol0.5mL, covered the PTFE film and silicon rubber gasket on the bottle, used the gland to compact aluminum cover, then sealed and mixed, put the sample into the constant temperature water bath with70℃for12min, then took the upper gas1mL under the120℃column temperature to detect. The analysis results showed that the recovery rate of the method was93%～97%, the relative standard deviation<2%. The method did not require automatic headspace sampler device, so that it can provide a method for those agencies and laboratories which were lack of equipment configuration and experimental conditions to detect the alcohol content in the blood. The whole test can be completed within a short time, the operation was simple and low cost, and the linear range, sensitivity and the recoveries could meet testing requirements. Use this method to discuss the blood preservation conditions. The results showed that if the extracted blood samples cannot be detected in time, it must be sealed and stored in the refrigerator. And the detection must be completed within a week. The direct injection gas chromatographic internal and external standard method for the determination alcohol content in blood was established. Matrix effect and the application of single point calibration method in practical work were discussed. The results showed that direct injection gas chromatography internal and external standard method could both meet the inspection requirements and there was no significant difference between them; there was no significant difference between blank whole blood for control and ethanol standard solution for control of the measured ethanol content, so the matrix effect could be ignored; single point calibration method was simple, rapid, and could be applied to the detection of a large number of samples in the actual work.The method for simultaneous determination of five trace elements (magnesium, calcium, iron, copper and zinc) in the serum was established. The select of analytical line of the five trace element was discussed. Through the discuss and selection of a range of instruments measuring conditions, the best experimental method for simultaneous determination of five trace elements (magnesium, calcium, iron, copper and zinc) in the serum was finally established. The results showed that the method had low detection limit(magnesium was3.03×10-4mg/L; calcium was1.21×10-3mg/L; iron was1.41×10-3mg/L; copper was5.00×10-3mg/L; zinc was1.13×10-3mg/L), recoveries ranged from95%to101%, and the relative standard deviation was less than3%. The method was simple, rapid, sensitivity, high accuracy and less interference.The method for determination of selenium in the blood was established. Adopting hydride generation combined with ICP-AES, the pre-reduction time and temperature were discussed. Through the discuss and selection of a range of instruments measuring conditions, the best experimental method for Hydride Generation-ICP-AES determination of selenium content in blood was finally established. The results showed that the detection limit of the method was0.0018μg/mL, recoveries ranged from95%to107%, and the relative standard deviation was less than3%. The method was simple, rapid, sensitivity, high accuracy and less interference. There were24figures,24tables and104references. |
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