Potentiometric Immunosensors Based On Prussian Blue Nanoparticles Modifided Carbon Paste Electrode

Immunosensor has fast response, high sensitivity and good selectivity.It has broad application prospects in the field of clinical testing, environmental science, food safety, and military medicines. In recent years, there are many types of immunesensors advent, the potentiometric immunosensor is the simplest and most practical one. The potentiometric immunosensor no enzyme-labeled antibody (or antigen),like ion selective electrode for rapid detection of antibody (or antigen). The equipment preparation detect low-cost, easy to implement miniaturization, the detection process does not require other reagents. However, earlier studies showed that the lower the detection sensitivity of the immune sensor, signal stability, thus few studies. With the rapid development of nanotechnology, nanomaterials application provides a new way to address the problems of the potentiometric immunosensor. In this paper, electropolymerization, eletrodeposition and self-assembled technology were used to immobilize some nanomaterials on the electrode surface.The use of the namomaterials not only improve the performance of the immunosensor but also provide a new subject for biosensor research field.1Potentiometric immunosensor based on gold-coated magnetic nanoparticlesPrussian blue nanoparticles were prepared by using Fe3O4superparamagnetic, and two kinds of nano materials were synthesized:Au@MFe3O4andAu@MPB.Au@MPB and Au@MFe3O4modified on carbon paste electrode by potentiostatic deposition respectively to form a potentiometric immunosensor. The resulting sensor showed simple preparation, low cost, short response time, and good reproducibility. After store20days later,the slope of the immunosensor was decreased by11.8%.The studied immunosensor exhibited a good stability.2Potentiometric immunosensor based on Polymerized O-phenylenediamine-colloid gold-prussian blue composite filmFirst,the o-phenylenediamine(OPD) was polymerized on a carbon paste electrode(CPE) by cyclic voltammetry method.Then the colloid gold and prussian blue were immobilized onto the CPE/POPD by eletrodeposition technology to fabricate CPE/POPD/GNPs-PB.Finally,the goat anti-mouse IgG(antibody,Ab) were self-assembled noto the PCEs through the Au-NH-bond to form a potentiometric immunosensor(CPE/POPD/GNPs-PB/Ab). The resulting immunosensor exhibited fast potentiometric response(<3min) under the antigen concentration of2.0×10-5ng/mL and1.0×104ng/mL. The studied immunosensor exhibited a short response time, high sensitivity,wide detection range and low cost. 3Potentiometric immunosensor based on gold nanoparticles and prussian blue-gold nanocomposite filmsFirst, gold nanoparticles was eletrodeposition on the carbon paste electrode(CPE) by cyclic voltammetry method.Then goat anti-mouse IgG(antibody,Ab) were self-assembled noto the electrode through the Au-NH2bond to form a simple potentiometric immunosensor (s-Ab/GNPs/CPE). The studied immunosensor exhibited a high sensitivity,wide linear range and low detection limit. Secondly, prussian blue-gold nanocomposite films and gold nanoparticles were modified on the carbon paste electrode(s-Ab/GNPs/PB@Au/CPE).Five immunosensors exhibited good reproducibility.Compared with Ab/GNPs/CPE,the sensitivity was improved, response time was shorten. The immunosensor will have a more promising application. Finally,protein A was immobilized onto the GNPs/PB@Au/CPE to achieve the oriented immobilization of rabbit IgG.A ptentiometric immunosensor was fabricated(r-Ab/PA/GNPs/PB@Au/CPE). Compared with s-Ab/GNPs/PB@Au/CPE,the studied immunosonser exhibited higher sensitivity and lower detection limit, and will have a wide range of application value in clinical detection.