2-de Based Comparative Proteomic Analysis Of Plasma From BD Patients And Healthy Controls

BackgroundBipolar disorder is a common psychiatric mood disorder affectingapproximately3.5%of the general population. There is no objectivelaboratory-based test available to aid BD diagnosis or monitor BDprogression, and little is known about BD’s underlying molecular basis.The identification of underlying molecular alterations should lead to animproved understanding of BD’s pathogenesis, so a comparativeproteomic study was performed to identify differentially expressedplasma proteins across various BD mood states (depressed BD, manic BD)relative to healthy controls.MethodsPlasma samples from the three experimental groups wereimmunodepleted of seven high-abundance proteins, subjected toproteome-wide expression profiling by two-dimensional electrophoresis,and then analyzed by matrix-assisted laser desorption/ionization time offlight. Proteomic results were validated by immunoblotting and analyzedwith MetaCore. ResultsFrom a total of43identified proteins,22proteins were differentiallyexpressed in BD subjects relative to healthy controls in both mood state.In contrast,21proteins were differentially expressed in only one BDmood state as compared to healthy controls. MetaCore was used toconstruct hypothetical networks of these dysregulated proteins; thebiological processes centered around several pathophysiologicalprocesses with immunoregulation displaying the highest statisticalsignificance. Expression of apo A1、apo L1and CA-1in the plasma ofBD subjects were confirmed to be significantly dysregulatedby Westernblotting.ConclusionsDysregulated proteins reveal that BD maybe associated with earlyperturbations in immunoregulation and lipid metabolism. Expression ofapo A1、apo L1and CA-1in plasma may be a potential and favorablediagnostic biomarker for BD.This finding might provide the basis forfuture clinical diagnostic tests of patients with BD.