The Isolation And Extraction Of Spicy Component From Zanthoxylum Schinifolium Sieb.etZucc

This experiment focused on spicy component. The spicy components were first isolated, which were then used as standard substance to optimize the extraction process. This research suggested some theoretical evidences to study contains of spicy component and deep processing. The followings are the main results and conclusions.1. The isolation and structure characterization of spicy componentSpicy components were extracted by supercritical CO2, which were then decolorized by D152and isolated by silica gelcolumn chromatography, polyamide column chromatography and LH-20gel filtration. The structure of the isolations were analyzed by IR and MS. The eluents of silica gel column chromatography and polyamide column chromatography were determined, which are CCL2：CH3OH (10:0.8) for the eluent of silica gel column chromatography, and70%CH2OH2for the eluent of polyamide column chromatography. The result indicated that the purified product is hydroxy1-α-sanshool or hydroxy1-β-sanshool.2. Optimizing supercritical carbon dioxide extraction process of spicy component from Zanthoxylum schinifolium Sieb.etZucc by response surface methodologyThe effect of mesh number, extraction time, extraction temperature, extraction press and the temperature of separator I on spicy component and the amount of extraction were investigated. Based on the single-factor experiments, the Box-Behnken center-united experimental design principles for3factors were used to determine the experiments condition and the response surfaces analysis. The results are:within the tested level ranges, the impact of three process parameters on the weight of spicy component from big to small was extraction temperature, extraction press, extraction time in turn. The optimum extraction temperature was45℃and extraction pressure was29MPa, extraction time was89min, the pressure and temperature of separator I was12Mpa and30℃,the pressure and temperature of separator Ⅱ was8Mpa and37℃, and the pressure of rectifying column was5Mpa. Under the condition of optimum process, it is110mg from5g raw material by supercritical CO2separation. The extraction rate is2.2%.3. Optimizing ultrasound assisted extraction of spicy component from Zanthoxylum schinifolium Sieb.etZucc by response surface methodologyThe effect of solvent, mesh number, ultrasound time, ultrasonic power and the ratio of liquid to solid on A254were investigated. Based on the single-factor experiments,the Box-Behnken center-united experimental design principles for3factors were used to determine the experiments condition and the response surfaces analysis. Within the tested level ranges, the impact of three process parameters on A254from big to small was ultrasonic power, ultrasonic time, the ratio of liquid to solid. The optimum solvent was ether, mesh number was60, ultrasound time was40min, ultrasonic power was100W, the ratio of liquid to solid was1:6. Under the condition of optimum process, it is0.78mg from5g raw material by Ultrasonic assisted solvent extraction. The extraction rate is0.0156%.