Microbial Community Of Surface Sediment In Ancient Cana And Its Correlation With Environmental Factors

The ancient Canal (Zhenjiang section) as an important river flowing through urbandistrict, water is polluted in different degrees. But the treatment is not very obvious. Thestudy has accumulated the data on enzymology indexes of water environment, provided thereference to solving the problems of city river pollution, and revealed microbial communitydistribution characteristics and laws in the surface sediments of the ancient canal. Moreover,the theoretical basis was provided on bioremediation of ancient canal sediments anddevelopment of new biological treatment technology of river pollution.The surface sediment in the ancient canal of Zhen Jiang were chosen as the researchobject.The temporal and spatial variation of alkaline phosphatase activity, nitrate reductaseactivity, nitrite reductase activity and study on relativity between the enzyme activity andthe anion concentration of nitrogen and phosphorus in the ancient. The results showed thatthere were temporal and regional variations of three enzyme activities in the sedments. Theenzyme activities were higher in the dry period (December) with values of0.29mg/(g24h)to0.32mg/(g24h),0.13mg/(g24h) to0.19mg/(g24h),3.05mg/(g24h) to5.21mg/(g24h), respectively. In contrast，lower enzyme activities were detected in the floodperiod (May) with values of0.18mg/(g24h) to0.29mg/(g24h),0.08mg/(g24h) to0.14mg/(g24h),2.95mg/(g24h) to3.64mg/(g24h), respectively. Redundancy analysisresults show that the enzyme activities have larger difference in the dry period than the floodperiod. The activities were positive correlated to the relevant anion concentrations(p＜0.05).The nitrate reductase activity, nitrite reductase activity have significant effect on theoverlying water.4-Amino antipyrine direct spectrophotometric method was used to study the volatilephenols in the surface sediments of the ancient canal. As the whole, the concentration of thevolatile phenol in the dry season is higher than flood. As the region, the concentration is A＞B＞C. The study analysis the microbial community diversity and structure of the surfacesediment, were detected in16kinds of PLFAs, which from C13to C20.The results ofPLFAs analysis showed that total PLFAs biomass of the microbes in area A was the highest,most of which were the bacteria and fungi. Redundancy analysis demonstrated that PAHspollution degree has a certain effect on soil microbial community structure.DGGE patterns and heavy metals analysis showed that the content of heavy metal elements Cd, Cr, Cu, Ni, Pb, Zn exist differences of time and space, and serious pollution.Redundancy analysis showed that genetic diversity of the microbial community of thespatio-temporal difference is bigger.Heavy metals Cd, Cr, Cu, Pb, Zn and Ni for allsediment microbial structure are different.The phenol-degrading bacterium was screened from sediments. The srains with Thestrain using phenol as a sole carbon could grew well in high phenol concentrationwastewater. It was identified as Pseudomonas sp. according to16S rDNA sequenceanalysis.The result shows The phenol-degradable strain rates up to99%, it is lower than thenational discharge standard.