Formulation Of Plumbagin Loaded PLGA-PEG-Aptamer Nanoparticles For Prostatic Cancer Chemotherapy

Objective: To develop a new chemotherapy drug for prostate cancer, and study itsnano formulations.Methods: Synthesis of PLGA-b-PEG-COOH and determine it by1H NMR in CDCl3at300Hz. Preparation of plumbagin-loaded PLGA-b-PEG-COOH nanoparticles;Conjugation of Anti-PSMA antibody to the PLGA-b-PEG-COOH NPs. Improvements offormulation and process; Find a appropriate Lyoprotectant. Zetapotential,Size andsize distribution of the prepared nanoparticles were measured by the LLS. drugcontent were Determination by HPLC.Surface morphology were imaged by a fieldemission scanning electron microscopy (FESEM) system and a transmission electronmicroscope(TEM). Drawing the in vitro drug release. T surface element was analysedby X-ray photoelectron spectroscopy (XPS).Results: The polymer were a mixture of PLGA-COOH and PLGA-PEG-COOH with amolar ratio of1:2.We get the optimal prescription of nano formulations. theresulting nanoparticles had a diameter of189.4±30.6nm, the zeta potential ofnanoparticles was17.1±3.7mV. the nanoparticles morphology is spherical, relativelyuniform size. The in vitro release profile of plumbagin-loaded PLGA-b-PEG-COOHnanoparticles is up to a week. XPS analysis showed that the surface of thenanoparticles with nitrogen, indicating the success of the antibody link.Conclusion: plumbagin-loaded PLGA-b-PEG-COOH nanoparticlescan be prepared bythe nanoprecipitation method. If a fat-soluble drug was slightly soluble in water,the nanoprecipitation method will lead to poor drug loading and poor encapsulationefficiency. water molecules can run into the pores of nanoparticle and dissolve thesekinds of drugs, so short its sustained release. The Conjugation of Ab to thePLGA-b-PEG-COOH NPs was performed by covalent binding under the catalysis of EDC/NHS.THE Ab equipped the nanopatical with Targeting.The active drug targetingsystem was successfully constructed, but the plumbagin is not as Charming asconventional drugs like docetaxel and cisplatin. Plumbagin is not an ideal drug forloading by nanoprecipitation method due to its physical properties...