| The sulfamonomethoxine is one of sulfonamide which is widely used in theprevention and treatment of birds diseases in a broad-spectrum antimicrobial range, and itswithdrawal time is up to28days. Sulfamonomethoxine is widely used in the treatment oflivestock diseases and as a feed additive used in the production of food of animalorigin.With the appearance of domestic vererinary drug abuse happenning frequently andthe awareness of Chinese food safety improving, the harm of veterinary drug residues tothe environment and the human body is paid attention by people. The big-bone broilerchichen in one day age were used in this study.160healthy big-bone broiler chichen fed bythe basal diet to40days of age were randomLy divided into four groups, each group of40.Since the beginning of41th days of age, the control group was only fed the basal diet, andlow-dose,medium-dose and high-dose group of the sulfamonomethoxine were fed the basaldiet respectively added the25mg/kg,200mg/kg and500mg/kg. They were fed for7dayscontinuously, ingestion and drinking water an will.Some chicken legs wew took randomLy in1d,4d,7d,10d,13d and16d after drugwithdrawal, the protein, fat, mineral element content and sulfamonomethoxine residueswere detected. By studying that the different doses and the drug residual had an impact onprotein, fat and mineral elements in the broiler body, and exploring related mechanism ofthis drug influence on the broiler body of this three anabolic nutritional elements, it couldprovide the necessary experimental data reference for drug safety evaluation, and make aforecast of the potential chronic hazards for human health, especially fot the protein, fatand mineral elements of human body’s metabolism and synthetic.1. Establishment of High-performance Liquid Chromatography for the determinationof sulfamonomethoxine residuce in chicken muscle According to the EU2002/657/EC ahigh performance liquid chromatography method for the determination of sulfamonomethoxine residue in chicken muscle was developed. The mobile phase was0.01mol/L ammonium acetate pH4.6(80%) and acetonitrile (20%). The homogenated samplewas extracted by liquid-liquid method using acetonitrile and n-hexane. Via SPE solidphase extraction the extraction was dried by the rotary evaporators, dissolved inacetonitrile, and filtered ration0.45μm microporefilm.over-4.5μm. The final solution wasanalyzed by HPLC. The decision limit of the method (CCα) was104.44μg/kg and thedetection capability (CCβ) was108.89μg/kg. The resulting correlation coefficients(r) of alllinear standard curves were greater than0.9997with the linear range of0.5~10μg/mL,good recovery84%was determined and the coefficient of variation was less than5%. Thelimit of detection and quantification limit were respectively0.15μg/kg and05μg/kg Itshows detection results of this developed method were accurate and reliable with withgood reproducibility, low cost and simple operation. Through testing the chicken leg meatof sulfamonomethoxine residuce, it could be below the EU limit requirement after10daysin7days under not more than500mg/kg administration.2. Relevant nutrients elements changing in chicken under the different dose. Theprotein content was significantly different (P＜0.01) among three dose groups by the effectof sulfamonomethoxine, magnesium content was different statistical level (P＜0.05) andthe fat, iron, copper, zinc and calcium content no statistically significant difference (P＞0.05). Compared with the control group, the high dose group of differences in proteins andfat content was significantly (P<0.05), differences in iron content was very significantly(P<0.01). Differences in iron content of the medium dose group was significantly(P<0.05), calcium very significant difference (P<0.01). Low-dose group of differences inprotein and magnesium content was significantly different (P<0.05). Conclusion:Sulfamonomethoxine residue can affect the related nutritional quality of chicken muscle ina dose-dependent manner. The protein and magnesium levels were increased under lowdose. The iron level was decreaed, but calcium increased under middle dose. The protein,fat and iron levels were all reduced under high dose. |
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