Microbial Community Structure And Function Of Drinking Water Reservoir

Reservoirs had been polluted more and more setiously and eutrophicationproblem is more prominent. In recent years, domestic and foreign scholars had usedvarious methods for micro-polluted water treatment. Biological treatment aroused wideconcern, which is treated as a cost-effective method to remove nitrogen from surfacewater. Based on the preliminary study in our group, aerobic denitrifiers were isolatedfrom sediment of the reservoirs and studied for nitrogen removal performance andspecies identification. The functional groups of sediment and water were studied. Theresults provide a scientific basis for the applied research of microbial remediation inthe micro-polluted water reservoir. The main results of this study are as follows:Firstly,234oligotrophic aerobic denitrifiers were isolated from sediment of theShibianyu reservoir in Xi’an and Zhoucun reservoir in Zaozhuang. In pure cultureconditions, there were126oligotrophic aerobic denitrifiers and nitrofen removal rate isover40%.The number of the strains of80%nitrogen removal rate in48h is8. Theresults of PCR Amplification sequencing by molecular biology techniques16S rDNAindicated that the effective strains are Acinetobacter, Ralstonia, Methylophilus,Rhizobium, Pseudomonas, Sphingomonas, Lysobacter and so on. Rhizobium as anitrogen-fixing microorganism is first found to be having the characteristics of aerobicdenitrification. The nitrogen removal of effective strains proved the impact of thetemperature,and the deniteifier HH12had the highest nitrogen removal rate of70%in30℃.Secdondly, the study had discussed the influence of indigenous microorganismpopulation diversity by the addition of efficient strain HF3. The results showed that, these are no significant impact by inoculating the efficient aerobic denitrifying bacteriaHF3on bacterial, fungal, actinomycic and sulfate-reducing bacterial group diversity. Itindicated that the efficient aerobic denitrifying bacteria HF3had no significant effecton ecological flora structure and had high ecological satety.Thirdly, enzyme activity and diversity of microbial community structure ofZhoucun reservoir sediments was studied by using the BIOLOG, PCR-DGGE andcloning sequencing methods. The results showed that the urease and protease activityof the sampling points A and F is2.48mg NH3-N/(g·24h) and16.28mgNH2-N/(g·24h),which are the lowest. The difference in urease and protease activity ofeach sampling point was significant. Sampling point richness index and diversity isbasically the same. Whether AWCD richness index or diversity index, sampling point Bsediments microorganisms were higher than other sampling points, and sampling pointE is the lowest. So, diversity of microbial community structure of of Zhoucun reservoirsediments had spatial heterofeneity.Fourthly, the result of micro-organisms diversity detection of Jinpen waterreservoir showed that in the nine different sampling points, the water micro-organismscarbon source consumption up to a maximum rate at samples A30m. It could be seenrich in diversity of each sampling sites of microbial population structure from theDGGE pictures. But the difference between the sample points was not very significantand by correlation analysis get the coupling mechanism of sediment microbialdiversity and water quality indicators：Depth and nitrite nitrogen significantly affectedthe vertical distribution of bacterial populations; nitrate nitrogen and ORP significantlyaffected the fungal populations vertical distribution;ammonia, nitrate, TOC and ORPsignificantly affected the actinomyces vertical distribution; nitrite nitrogensignificantly affected the sulfate-reducing bacterial community of vertical distributionin the drinking water reservoir. So, functional microbial structure of drinking waterwas influenced by various water quality index.